Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INCREASING HYPOTHALAMIC ARCUATE NUCLEUS GLIAL PEROXIDASE-ACTIVITY IN AGING FEMALE RATS IS REDUCED BY AN ANTIESTROGEN AND A GONADOTROPIN-RELEASING-HORMONE AGONIST
Autore:
SEIFER DB; ROAPENA L; KEEFE DL; ZHANG HP; GOODMAN S; JONES EE; NAFTOLIN F;
Indirizzi:
BROWN UNIV,WOMEN & INFANTS HOSP,SCH MED,DEPT OBSTET & GYNECOL,DIV REPROD ENDOCRINOL PROVIDENCE RI 02905 YALE UNIV,SCH MED,DEPT OBSTET & GYNECOL NEW HAVEN CT 00000 YALE UNIV,SCH MED,DEPT EPIDEMIOL NEW HAVEN CT 00000
Titolo Testata:
Menopause
fascicolo: 2, volume: 1, anno: 1994,
pagine: 83 - 90
SICI:
1072-3714(1994)1:2<83:IHANGP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PITUITARY GONADOTROPHS; REPRODUCTIVE FUNCTION; LUTEINIZING-HORMONE; ESTRADIOL VALERATE; ESTROUS CYCLICITY; PREOPTIC AREA; BRAIN; OVARIAN; KEOXIFENE; YOUNG;
Keywords:
DAB; KEOXIPHENE; GOSERELIN; ARCUATE NUCLEUS; GLIAL PEROXIDASE ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
D.B. Seifer et al., "INCREASING HYPOTHALAMIC ARCUATE NUCLEUS GLIAL PEROXIDASE-ACTIVITY IN AGING FEMALE RATS IS REDUCED BY AN ANTIESTROGEN AND A GONADOTROPIN-RELEASING-HORMONE AGONIST", Menopause, 1(2), 1994, pp. 83-90

Abstract

Progressive arcuate glial peroxidase activity is associated with reproductive aging of the female rat. We tested the hypothesis that age-related increase of glial peroxidase activity within the arcuate nucleusand posterior periventricular area is due to endogenous estrogen and could be reduced by an antiestrogen, keoxiphene, or a gonadotropin-releasing hormone super agonist, goserelin acetate (Zoladex), or both. At3 months of age, 4-5-day regularly cycling female rats were divided into four groups. One group served as a baseline 3-month-old control and was killed by perfusion-fixation followed by serial sectioning of the hypothalamus and staining with 3,3'-diaminobenzidine tetrahydrochloride (DAB). Other groups consisted of animals receiving keoxiphene, goserelin acetate depot (Zoladex), or sham implants for 6 months. Two months after removal of the implants, at 11.25 months of age, the animalswere killed by perfusion-fixation, and serial hypothalamic sections were stained with DAB. Sections from each of the four groups were examined in a fixed-size standard test area using a computerized image analyzer to assess the surface density of the DAB reaction. Overall, periventricular peroxidase reaction of the 11.25-month keoxiphene group showed a 63.5% increase in surface density of DAB reaction over the 3-month control group (5,834.6 +/- 101.0 mu m(2) versus 3,566.2 +/- 173.6 mu m(2)), whereas the 11.25-month Zoladex group showed a 42.4% increasein surface density of DAB reaction over the 3-month control group (5,078.5 +/- 114.6 mu m(2) versus 3,566.2 +/- 173.6 mu m(2)). This was insharp contrast to the 11.25 month sham control group, which showed a 94% increase over the 3-month control group (6,926.6 +/- 121.3 mu m(2)versus 3,566.2 +/- 173.6 mu m(2)), p < 0.0001. The differences in increase of the surface density of DAB in zone of reaction in keoxiphene-treated and Zoladex-treated rats were not equally distributed throughout the periventricular brain: the greatest difference was noted in theanterior arcuate (p < 0.0001), followed by the posterior arcuate (p <0.001), with no difference noted in the posterior periventricular area. These results demonstrate than an antiestrogen and functional hypogonadotropic hypogonadism induced by a gonadotropin-releasing hormone agonist reduce the increase in hypothalamic glial peroxidase activity in the arcuate nucleus of maturing (aging) female rats but not in the posterior periventricular area. They support the hypothesis that estrogen secreted during the ovarian cycle contributes causally to hypothalamic aging in the female rat and indicate the presence of an estrogen-sensitive anterior region and an estrogen-insensitive posterior periventricular zone of age-dependent glial reaction. The failure of blockadeby Zoladex further indicates that hypothalamic aging in the posteriorperiventricular area may be indifferent to the presence of the ovary.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 07:43:58