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Titolo:
EXPRESSION OF P1 DNA IN MAMMALIAN-CELLS AND TRANSGENIC MICE
Autore:
MCCORMICK SPA; LINTON MF; YOUNG SG;
Indirizzi:
UNIV CALIF SAN FRANCISCO,GLADSTONE INST CARDIOVASC DIS,POB 419100 SANFRANCISCO CA 94141 UNIV CALIF SAN FRANCISCO,GLADSTONE INST CARDIOVASC DIS SAN FRANCISCO CA 94141 UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST SAN FRANCISCO CA 94143 VANDERBILT UNIV,DIV ENDOCRINOL NASHVILLE TN 00000
Titolo Testata:
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING
fascicolo: 5-6, volume: 11, anno: 1994,
pagine: 158 - 164
SICI:
1050-3862(1994)11:5-6<158:EOPDIM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN APOLIPOPROTEIN B100; MESSENGER-RNA; CLONING; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
S.P.A. Mccormick et al., "EXPRESSION OF P1 DNA IN MAMMALIAN-CELLS AND TRANSGENIC MICE", GENET A-BIO, 11(5-6), 1994, pp. 158-164

Abstract

Because the P1 bacteriophage packages DNA inserts of 80-100 kb, whichare much larger than inserts of bacteriophage lambda or cosmid vectors, P1 DNA can be used to express large genes in cultured cells and transgenic mice. We obtained a P1 bacteriophage clone with a 79.5-kb insert (p158) that spanned the entire human apolipoprotein (apo-) B gene. We used the insert from p158 to express the human apo-B gene in both cultured rat hepatoma cells and transgenic mice. In this article, we review our apo-B expression studies and discuss the techniques that we have used for these expression studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:39:46