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Titolo:
COMPLEX ORGANIZATION OF CTF NF-I, C/EBP, AND HNF3 BINDING SITES WITHIN THE PROMOTER OF THE LIVER-SPECIFIC VITELLOGENIN GENE/
Autore:
CARDINAUX JR; CHAPEL S; WAHLI W;
Indirizzi:
UNIV LAUSANNE,INST BIOL ANIM,BATIMENT BIOL CH-1015 LAUSANNE SWITZERLAND UNIV LAUSANNE,INST BIOL ANIM CH-1015 LAUSANNE SWITZERLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 52, volume: 269, anno: 1994,
pagine: 32947 - 32956
SICI:
0021-9258(1994)269:52<32947:COOCNC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEROID-DEPENDENT INTERACTION; ESTROGEN-RESPONSIVE ELEMENT; TUMOR VIRUS PROMOTER; XENOPUS-LAEVIS; TRANSCRIPTION FACTORS; INVITRO TRANSCRIPTION; NUCLEAR FACTOR; YOLK PROTEINS; DNA-BINDING; RAT-LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
J.R. Cardinaux et al., "COMPLEX ORGANIZATION OF CTF NF-I, C/EBP, AND HNF3 BINDING SITES WITHIN THE PROMOTER OF THE LIVER-SPECIFIC VITELLOGENIN GENE/", The Journal of biological chemistry, 269(52), 1994, pp. 32947-32956

Abstract

Vitellogenin genes are expressed specifically in the liver of female oviparous vertebrates under the strict control of estrogen. To explainthis tissue-specific expression, we performed a detailed analysis of the Xenopus laevis vitellogenin gene B1 promoter by DNase I footprinting and gel mobility-shift assays. We characterized five binding sites for the ubiquitous factor CTF/NF-I. Two of these sites are close to the TATA-box, whereas the others are located on both sides of the estrogen responsive unit formed by two imperfect estrogen response elements. Moreover two liver-enriched factors, C/EBP and HNF3, were found to interact with multiple closely spaced proximal promoter elements in the first 100 base pairs upstream of the TATA-box. To confirm the physiological significance of this in vitro analysis, in vivo DNase I footprinting experiments were carried out using the ligation-mediated polymerase chain reaction technique. The various cis-elements characterized invitro as binding sites for known transcription factors and more particularly for liver enriched transcription factors are efficiently recognized in vivo as well, suggesting that they play an important role in the control of the liver-specific vitellogenin gene B1 expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:06:40