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Titolo:
GLUCURONIDASE EXPRESSION IN TRANSGENIC TOBACCO ROOTS WITH A PARASPONIA PROMOTER ON INFECTION WITH MELOIDOGYNE-JAVANICA
Autore:
EHSANPOUR AA; JONES MGK;
Indirizzi:
ESFAHAN UNIV,INST HORT,DEPT BIOL ESFAHAN IRAN MURDOCH UNIV,DEPT PLANT SCI PERTH WA 6150 AUSTRALIA MURDOCH UNIV,SCH BIOL & ENVIRONM SCI,STATE AGR BIOTECHNOL CTR PERTH WA 6150 AUSTRALIA
Titolo Testata:
Journal of nematology
fascicolo: 4, volume: 28, anno: 1996,
pagine: 407 - 413
SICI:
0022-300X(1996)28:4<407:GEITTR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
KNOT NEMATODE; HEMOGLOBIN GENES; PLANTS; TRANSCRIPTS; STRATEGIES;
Keywords:
DOWN-REGULATION; GENE EXPRESSION; GLUCURONIDASE; MELOIDOGYNE JAVANICA; NEMATODE; NICOTIANA TABACUM; PARASPONIA ANDERSONII; ROOT-KNOT NEMATODE; TOBACCO; UP-REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
A.A. Ehsanpour e M.G.K. Jones, "GLUCURONIDASE EXPRESSION IN TRANSGENIC TOBACCO ROOTS WITH A PARASPONIA PROMOTER ON INFECTION WITH MELOIDOGYNE-JAVANICA", Journal of nematology, 28(4), 1996, pp. 407-413

Abstract

The expression of a gus reporter gene linked to a Parasponia andersonii hemoglobin promoter has been studied in transgenic tobacco plants after infection by Meloidogyne javanica. Transgenic roots were harvested at different times after nematode inoculation, and stained histochemically for expression of the gus gene. During the early stages of infection (0-2 weeks) there was little expression in giant cells, in contrast to other cells of the root. In later stages of infection (3-6 weeks) there was strong gus expression in giant cells, with virtually no expression in other cells of the root. The Parasponia hemoglobin promoter therefore appears to direct down-regulation of linked genes on induction of giant cells, but up-regulation in mature giant cells. This reflects different metabolic activities in the giant cells depending on their stage of development. The Parasponia hemoglobin promoter may respond to oxygen tension in giant cells. This suggests that oxygen tension may be limited in the metabolically active giant cells that are associated with egg-laying females.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:53:44