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Titolo:
REDUCED DIMERIZATION OF LIPOPROTEIN-LIPASE IN POSTHEPARIN PLASMA OF APATIENT WITH HYPERCHYLOMICRONEMIA
Autore:
MASUNO H; NAKABAYASHI H; KOBAYASHI J; SAITO Y; OKUDA H;
Indirizzi:
EHIME COLL HLTH SCI,DEPT MED LAB TECHNOL TOBE EHIME 79121 JAPAN NIHON UNIV,SCH MED,MED RES INST,ITABASHI KU TOKYO 173 JAPAN CHIBA UNIV,SCH MED,DEPT INTERNAL MED 2 CHIBA 280 JAPAN EHIME UNIV,SCH MED,DEPT MED BIOCHEM SHIGENOBU EHIME 79102 JAPAN
Titolo Testata:
Biochimica et biophysica acta, L. Lipids and lipid metabolism
fascicolo: 1, volume: 1254, anno: 1995,
pagine: 30 - 36
SICI:
0005-2760(1995)1254:1<30:RDOLIP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN POSTHEPARIN PLASMA; RADIATION INACTIVATION; DEFICIENCY CLD; MOUSE; ADIPOCYTES; MUTATION; BINDING; SIZE; MICE;
Keywords:
LIPOPROTEIN LIPASE; AGGREGATION; MONOMER; DIMER; HYPERCHYLOMICRONEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
H. Masuno et al., "REDUCED DIMERIZATION OF LIPOPROTEIN-LIPASE IN POSTHEPARIN PLASMA OF APATIENT WITH HYPERCHYLOMICRONEMIA", Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1254(1), 1995, pp. 30-36

Abstract

As in post-heparin plasma of control subjects, post-heparin plasma ofa patient with hyperchylomicronemia contained lipoprotein lipase (LPL) subunits with M(r) = 57000. But although the amount of LPL was the same as in post-heparin plasma of controls, no LPL activity was detectable. Nearly all the LPL in post-heparin plasma of controls bound to heparin-Sepharose and this LPL bound was mainly eluted with 1.5 M NaCl in parallel with the activity. In post-heparin plasma of the patient, 58% of the LPL subunits did not bind to heparin-Sepharose and 23% was eluted with 0.6 M NaCl. Studies by sucrose density gradient centrifugation showed that almost all the LPL in post-heparin plasma of controls was recovered in the peak with a sedimentation coefficient of 6.8 S, corresponding to the position of a dimeric form of LPL, in parallel with the activity; little LPL was recovered in the peak with a sedimentation coefficient of 4.0 S, corresponding to the position of a monomericform of LPL. In post-heparin plasma of the patient, 35% of the LPL subunits was recovered in fractions with larger sedimentation coefficients at the bottom of the centrifuge tube, indicating the presence of anaggregated form(s) of LPL; the amount of the monomeric form of LPL was increased, while that of the dimeric form was decreased. Thus, defect of LPL activity in post-heparin plasma of the patient with hyperchylomicronemia could result from reduced dimerization of LPL subunits.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:28:34