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Titolo:
IMAGE-ANALYSIS OF LYSOSOMAL ACTIVITY DURING THE EARLY CLONAL LIFE OF PARAMECIUM-PRIMAURELIA
Autore:
RAMOINO P; BELTRAME F; FATO M;
Indirizzi:
UNIV GENOA,INST ZOOL,VIA BALBI 5 I-16126 GENOA ITALY UNIV GENOA,DEPT COMMUN COMP & SYST SCI I-16145 GENOA ITALY
Titolo Testata:
FEMS microbiology letters
fascicolo: 1, volume: 125, anno: 1995,
pagine: 57 - 61
SICI:
0378-1097(1995)125:1<57:IOLADT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAUDATUM;
Keywords:
ACID PHOSPHATASE ACTIVITY; FOOD VACUOLES; CLONAL LIFE; CITOMETRY; CELL IMAGE ANALYSIS; PARAMECIUM PRIMAURELIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
P. Ramoino et al., "IMAGE-ANALYSIS OF LYSOSOMAL ACTIVITY DURING THE EARLY CLONAL LIFE OF PARAMECIUM-PRIMAURELIA", FEMS microbiology letters, 125(1), 1995, pp. 57-61

Abstract

Acid phosphatase activity was measured in individual cells by determining their optical densities through a scanning confocal laser microscope. The naphthol AS-TR (3-hydroxy-2-naphtoic acid 4'-chloro-2'-methylanilide) phosphate-hexazotized para-rosanilin method was used to visualise the acid phosphatase content in the light microscope. Evidence was obtained that the amount of enzyme varied in exponential growth phase cells as the fission age increased. By comparing the acid phosphatase activity with the rate of food vacuole formation, it appeared that the amount of enzyme inside the cells decreased in early clonal life, whereas the rate of food uptake increased. It was assumed that the reduction of acid phosphatase content could lead to a more extended life of vacuoles and to a decreased membrane recycling rate. In turn, the reduced supply of membrane available for food vacuole formation could partly be responsible for the decrease of the food uptake rate observed after the initial increase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 23:32:22