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Titolo:
VITRIFICATION OF AQUEOUS SUSPENSIONS FROM A CONTROLLED ENVIRONMENT FOR ELECTRON-MICROSCOPY - AN IMPROVED PLUNGE COOLING DEVICE
Autore:
BATTERSBY BJ; SHARP JCW; WEBB RI; BARNES GT;
Indirizzi:
UNIV QUEENSLAND,DEPT CHEM ST LUCIA QLD 4072 AUSTRALIA UNIV QUEENSLAND,CTR MICROSCOPY & MICROANAL ST LUCIA QLD 4072 AUSTRALIA
Titolo Testata:
Journal of Microscopy
, volume: 176, anno: 1994,
parte:, 2
pagine: 110 - 120
SICI:
0022-2720(1994)176:<110:VOASFA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
DRYING-INDUCED ARTIFACTS; SURFACTANT DISPERSIONS; BIOLOGICAL SPECIMENS; WATER;
Keywords:
CRYOELECTRON MICROSCOPY; VITRIFICATION; VITREOUS ICE; PHOSPHOLIPID; VESICLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
B.J. Battersby et al., "VITRIFICATION OF AQUEOUS SUSPENSIONS FROM A CONTROLLED ENVIRONMENT FOR ELECTRON-MICROSCOPY - AN IMPROVED PLUNGE COOLING DEVICE", Journal of Microscopy, 176, 1994, pp. 110-120

Abstract

In the process of vitrifying aqueous suspensions for cryo-transmission electron microscopy, water is solidified without crystallization. Vitrification can be achieved by rapidly plunging an aqueous thin film into a liquid cryogen. The preparation of aqueous thin films prior to vitrification must be performed in an environmental cabinet at controlled temperature and humidity in order to prevent evaporation and temperature-induced phase changes in the thin film. The device described here incorporates several important features which make the apparatus simpler and more convenient to use than similar devices described in the literature. One of these features includes the use of a totally enclosed environmental cabinet in which the grid, sample, micropipette and absorbent paper are equilibrated before thin-film preparation. Other features include a cryogen dewar on a swing arm for easy refilling, a guillotine shutter which is used to trigger the plunger electrically anda semiautomatic system which facilitates rapid transfer of the vitrified specimen from liquid propane to liquid nitrogen for storage and reduces handling of the specimen. To demonstrate the utility of the device, results showing the influence of temperature on the morphology of phospholipid vesicles are presented. A commercial cryotransfer apparatus (which is used for transportation of the vitrified specimen to the electron microscope cold-stage) has been modified to reduce the possibility of reversion of the Vitreous phase to the crystalline ice phases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:23:39