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Titolo:
CORTICOSTERONE MODULATES GROWTH HORMONE-RELEASING FACTOR AND SOMATOSTATIN IN FETAL-RAT HYPOTHALAMIC CULTURES
Autore:
FERNANDEZVAZQUEZ G; CACICEDO L; LORENZO MJ; TOLON R; LOPEZ J; SANCHEZFRANCO F;
Indirizzi:
CTR NACL INVEST CLIN,INST SALUD CARLOS 3,SERV ENDOCRINOL,SINESIO DELGADO 10 E-28029 MADRID SPAIN CTR NACL INVEST CLIN,INST SALUD CARLOS 3,SERV ENDOCRINOL E-28029 MADRID SPAIN HOSP RAMON Y CAJAL,SERV ENDOCRINOL E-28034 MADRID SPAIN
Titolo Testata:
Neuroendocrinology
fascicolo: 1, volume: 61, anno: 1995,
pagine: 31 - 35
SICI:
0028-3835(1995)61:1<31:CMGHFA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; GLUCOCORTICOID RECEPTOR; CUSHINGS-SYNDROME; GH SECRETION; DEXAMETHASONE; INVITRO; SOMATOMEDIN; DISEASE; CELLS; BRAIN;
Keywords:
CORTICOSTERONE; GROWTH HORMONE-RELEASING HORMONE; SOMATOSTATIN; NEURONAL CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
G. Fernandezvazquez et al., "CORTICOSTERONE MODULATES GROWTH HORMONE-RELEASING FACTOR AND SOMATOSTATIN IN FETAL-RAT HYPOTHALAMIC CULTURES", Neuroendocrinology, 61(1), 1995, pp. 31-35

Abstract

It is well known that chronic supraphysiological doses of glucocorticoids (GC) inhibit GH secretion in vivo, and stimulate GH secretion from the somatotropes in vitro. It has been suggested that GC exert an inhibitory role in the hypothalamus surpassing the GC-positive effect atthe somatotrope level. To test the hypothesis that GC can affect growth hormone-releasing factor (GRF) and somatostatin (SS) at the hypothalamic level, we studied the effect of corticosterone on the immunoreactive content of GRF (IR-GRF) and SS (IR-SS) in cells and media of fetal hypothalamic cells in culture. After 20 days in culture, cells were incubated with serum-free medium containing corticosterone (from 0.3 to 300 nM) for 48 h. Corticosterone had a dual effect on IR-GRF. Concentrations in the range of the glucocorticoid receptor Kd (3 nM) increased peptide content, whereas higher concentrations (30 and 300 nM) decreased IR-GRF content in cells and media. Conversely, corticosterone increased SS cell content, only at a concentration of 3 nM, inducing a 2- to 3-fold increment in media content with the highest doses (30 and 300 nM). These results demonstrated that both GRF and SS are modulatedby corticosterone in primary fetal rat hypothalamic cultures. WhereasGRF exhibited a dual response, stimulatory and inhibitory, at low andhigh corticosterone doses, respectively, SS showed a parallel increase with the corticosterone concentrations.

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Documento generato il 20/09/20 alle ore 04:29:09