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Titolo:
CHARACTERIZATION OF CONNECTIN-LIKE PROTEINS OF OBLIQUELY STRIATED-MUSCLE OF A POLYCHAETE (ANNELIDA)
Autore:
KAWAMURA Y; SUZUKI J; KIMURA S; MARUYAMA K;
Indirizzi:
CHIBA UNIV,FAC SCI,DEPT BIOL CHIBA 263 JAPAN CHIBA UNIV,FAC SCI,DEPT BIOL CHIBA 263 JAPAN
Titolo Testata:
Journal of muscle research and cell motility
fascicolo: 6, volume: 15, anno: 1994,
pagine: 623 - 632
SICI:
0142-4319(1994)15:6<623:COCPOO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAENORHABDITIS-ELEGANS; INSECT FLIGHT; TITIN; IDENTIFICATION; LOCALIZATION; PURIFICATION; FILAMENTS; SEQUENCE; TWITCHIN; UNC-22;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
Y. Kawamura et al., "CHARACTERIZATION OF CONNECTIN-LIKE PROTEINS OF OBLIQUELY STRIATED-MUSCLE OF A POLYCHAETE (ANNELIDA)", Journal of muscle research and cell motility, 15(6), 1994, pp. 623-632

Abstract

In obliquely striated muscle of polychaete, neanthes sp., three kindsof connectin (titin)-like high molecular weight proteins, similar to 4000kDa, similar to 1200kDa and similar to 700kDa, were detected by SDS gel electrophoresis and immunoblots using antibodies to vertebrate skeletal muscle connectin and antiserum to the protein in question. The700kDa protein was isolated and characterized as a beta sheet-rich filament 170 nm long and 4 nm wide. Using polyclonal antibodies to the 700kDa protein, the binding of the immunogold to the thick filament wasonly demonstrated in high ionic strength relaxing solution which solubilized some myosin. This observation suggested that the 700kDa protein was localized below the layers of myosin in the thick filament and this localization is different from that of twitchin of C. elegans bodywall muscle that is on the surface of thick filament. The 4000kDa protein was identified as a very thin filament linking the thick filament to the dense body. The very thin filaments were visualized in gelsolin-treated actin filament-free fibres. The 1200kDa protein was located in the periphery of the dense body. A model of the elastic filament in polychaete bodywall muscle is presented.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 16:19:07