Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IMMUNE-RESPONSES BY CORD-BLOOD CELLS
Autore:
RONCAROLO MG; BIGLER M; CIUTI E; MARTINO S; TOVO PA;
Indirizzi:
DNAX RES INST MOLEC & CELLULAR BIOL INC,DEPT HUMAN IMMUNOL,901 CALIF AVE PALO ALTO CA 94304 UNIV TURIN,IST DISCIPLINE PEDIAT TURIN ITALY
Titolo Testata:
Blood cells
fascicolo: 2-3, volume: 20, anno: 1994,
pagine: 573 - 586
SICI:
0340-4684(1994)20:2-3<573:IBCC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERFERON-GAMMA; B-CELLS; INTERLEUKIN-4; LYMPHOCYTES; CLONES; IL-10; HOST;
Keywords:
HUMAN UMBILICAL CORD BLOOD; IMMUNE RESPONSES; GRAFT VS HOST DISEASE; CYTOKINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
M.G. Roncarolo et al., "IMMUNE-RESPONSES BY CORD-BLOOD CELLS", Blood cells, 20(2-3), 1994, pp. 573-586

Abstract

In the present study, the biological properties of cord blood cells were investigated. Cord blood mononuclear cells and T cells responded normally to activation by alloantigens in primary mixed leukocyte reactions (MLRs), indicating that cord blood T cells can be normally activated via their TcR and have normal proliferative capacities. In addition, they expressed normal levels of accessory molecules such as CD28 and LFA-1, which contribute to amplify their responses. In contrast, cord blood mononuclear cells, but not cord blood monocytes, had a reducedcapacity to stimulate allogeneic cells in primary MLRs, In addition, cord blood monocytes express lower levels of HLA-DR and ICAM-1 compared to adult peripheral blood monocytes. Cord blood mononuclear cells were also impaired in their capacity to generate allogeneic cytotoxic activity in primary mixed leukocyte cultures (MLCs). In contrast, cord blood B cells were similar to adult B cells in their capacity to switchto immunoglobulin E producing cells when incubated with interleukin-4(IL-4) and anti-CD40 monoclonal antibody. We also demonstrated that IL-2, IL-6, and tumor necrosis factor-alpha (TNF-alpha) production by activated cord blood mononuclear cells was comparable to that observed with peripheral blood mononuclear cells isolated from normal adult donors. In contrast, interferon-gamma (IFN-gamma) was significantly decreased, whereas IL-4 and IL-5 were absent. Granulocyte-macrophage colony-stimulating factor (GM-CSF) levels were in general higher in the supernatants of cord blood cells. Thus, cord blood immune responses differfrom those of peripheral blood at several levels. Whether these differences account for a reduced capacity of transplanted cord blood cellsto modulate graft vs. host disease remains to be determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 04:28:16