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Titolo:
ANALYSIS OF HUMAN HEMATOPOIETIC STEM-CELL POPULATIONS
Autore:
MURRAY L; DIGIUSTO D; CHEN B; CHEN S; COMBS J; CONTI A; GALY A; NEGRIN R; TRICOT G; TSUKAMOTO A;
Indirizzi:
SYSTEMIX INC,3155 PORTER DR PALO ALTO CA 94304 STANFORD UNIV,MED CTR STANFORD CA 94305 ARKANSAS CANC RES CTR LITTLE ROCK AR 00000
Titolo Testata:
Blood cells
fascicolo: 2-3, volume: 20, anno: 1994,
pagine: 364 - 370
SICI:
0340-4684(1994)20:2-3<364:AOHHSP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICE;
Keywords:
STEM CELL; SCID-HU; LONG-TERM CULTURE-INITIATING CELLS; THY-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
7
Recensione:
Indirizzi per estratti:
Citazione:
L. Murray et al., "ANALYSIS OF HUMAN HEMATOPOIETIC STEM-CELL POPULATIONS", Blood cells, 20(2-3), 1994, pp. 364-370

Abstract

Purification of human hematopoietic stem cells (HSC) may be useful clinically for preparation of tumor-free grafts to be used for autologous transplantation and as targets for gene therapy, To analyze the phenotype of the human HSC, assays were used that measure the unique properties of stem cells, i.e., their long-term repopulating ability and their multilineage potential, These assays include: (1) an in vitro long-term hematopoietic culture system, using the murine bone marrow stromal cell line SyS1, which supports both B lymphopoiesis and myelopoiesis; (2) fetal human bone grafts implanted in SCID-hu mice, in which maintenance of CD34(+) cells and B and myeloid differentiative capacity of candidate stem cell populations may be measured; (3) fetal human thymus grafts in SCID-hu mice, which allow the analysis of in vivo T-cellpotential of a candidate stem cell population, Stem cells in adult bone marrow (ABM) or cytokine-mobilized peripheral blood (MPB) are thought to express CD34 but lack expression of markers indicating lineage commitment, This CD34(+) Lineage(-) (Lin(-)) subpopulation has been isolated by fluorescence-activated cell sorting and tested for activity in the assays described here. CD34(+) Lin(-) cells from both ABM and MPB demonstrated long-term engraftment in the SCID-hu bone model, This CD34(+) Lin(-) population can be subfractionated further using an antibody to Thy-1. The Thy-(1+) subset of CD34(+) Lin(-) cells is enriched for both long-term culture-initiating cells (LTC-1C) and has the ability to engraft in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 03:56:09