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Titolo:
PRIMITIVE HEMATOPOIETIC PROGENITOR CELLS ARE PRESENT IN PERIPHERAL-BLOOD AUTOGRAFTS
Autore:
TONG J; GIANNI AM; SIENA S; SROUR EF; BREGNI M; HOFFMAN R;
Indirizzi:
SYSTEMIX INC,3155 PORTER DR PALO ALTO CA 94304 INDIANA UNIV,SCH MED,HEMATOL ONCOL SECT INDIANAPOLIS IN 00000 INST NAZL TUMORI,DIV MED ONCOL,CRISTINO GANDINI TRANSPLANT UNIT MILANITALY UNIV MILAN,INST MED SCI MILAN ITALY
Titolo Testata:
Blood cells
fascicolo: 2-3, volume: 20, anno: 1994,
pagine: 351 - 363
SICI:
0340-4684(1994)20:2-3<351:PHPCAP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; BONE-MARROW CELLS; C-KIT; STEM-CELLS; TRANSPLANTATION; INVITRO; CIRCULATION; GENERATION; EXPANSION;
Keywords:
PERIPHERAL BLOOD GRAFTS; HEMATOPOIETIC STEM CELLS; LONG-TERM CULTURE-INITIATING CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
J. Tong et al., "PRIMITIVE HEMATOPOIETIC PROGENITOR CELLS ARE PRESENT IN PERIPHERAL-BLOOD AUTOGRAFTS", Blood cells, 20(2-3), 1994, pp. 351-363

Abstract

In this report we have used an in vitro assay for long-term culture-initiating cells (LTC-IC) to detect primitive hematopoietic progenitor cells (HPC) in the peripheral blood (PB) of cancer patients who received high-dose cyclophosphamide (HD-CTX) followed by a combination of recombinant hematopoietic growth factors (C-HGF) including either interleukin-3 (IL-3) + granulocyte colony-stimulating factor (G-CSF), IL-3 granulocyte-macrophage colony-stimulating factor (GMCSF) or IL-3/GM-CSF fusion protein (PIXY-321). In addition, we have developed a quantitative assay for cells capable of generating additional colony-forming cells (pre-CFC) as a means of determining primitive HPC present in mobilized PB cells, CD34(+) human leukocyte A (HLA)-DR(-) cells isolated from the mobilized PB were capable of initiating long-term hematopoiesis in vitro that persisted for 10 weeks, while CD34(+) HLA-DR(-) cellsobtained from the nonmobilized PB or BM were capable of sustaining long-term hematopoiesis in vitro for only 4 weeks and 8 weeks,, respectively, As determined by a limiting dilution analysis of mobilized PB CD34 HLA-DR(-) cells, the frequency of pre-CFC was 4.3% (range, 1.0-8.3%), Pre-CFC comprised 0.01% (range, 0.001-0.02%) of mobilized PB mononuclear cells, and 151 pre-CFC were calculated to be present in one milliliter of mobilized PB (range, 20-310/ml), These results suggest that PB mononuclear cells collected by leukapheresis following mobilizationwith HD-CTX + C-HGFs contain not only differentiated HPCs but also more primitive HPC.

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Documento generato il 18/09/20 alle ore 10:55:24