Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
REVISED SEQUENCE OF THE PORPHYROMONAS-GINGIVALIS PRTT CYSTEINE PROTEASE HEMAGGLUTININ GENE - HOMOLOGY WITH STREPTOCOCCAL PYROGENIC EXOTOXIN-B STREPTOCOCCAL PROTEINASE
Autore:
MADDEN TE; CLARK VL; KURAMITSU HK;
Indirizzi:
VET ADM MED CTR,RES SECT 151C PORTLAND OR 97201 UNIV ROCHESTER,SCH MED & DENT,DEPT DENT RES ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED & DENT,DEPT MICROBIOL & IMMUNOL ROCHESTER NY 14642 SUNY BUFFALO,DEPT ORAL BIOL BUFFALO NY 14214
Titolo Testata:
Infection and immunity
fascicolo: 1, volume: 63, anno: 1995,
pagine: 238 - 247
SICI:
0019-9567(1995)63:1<238:RSOTPP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; BACTEROIDES-GINGIVALIS; MOLECULAR-CLONING; CARICA-PAPAYA; PURIFICATION; TOXIN; CDNA; ASACCHAROLYTICUS; MELANINOGENICUS; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
T.E. Madden et al., "REVISED SEQUENCE OF THE PORPHYROMONAS-GINGIVALIS PRTT CYSTEINE PROTEASE HEMAGGLUTININ GENE - HOMOLOGY WITH STREPTOCOCCAL PYROGENIC EXOTOXIN-B STREPTOCOCCAL PROTEINASE", Infection and immunity, 63(1), 1995, pp. 238-247

Abstract

The prtT gene from Porphyromonas gingivalis ATCC 53977 was previouslyisolated from an Escherichia coli clone possessing trypsinlike protease activity upstream of a region encoding hemagglutinin activity (J, Otogoto and H. Kuramitsu, Infect, Immun, 61;117-f23, 1993). Subsequent molecular analysis of this gene has revealed that the PrtT protein is larger than originally reported, encompassing the hemagglutination regio. Results of primer extension experiments indicate that the translation start site was originally misidentified. An alternate open readingframe of nearly 2.7 kb, which encodes a protein in the size range of 96 to 99 kDa, was identified. In vitro transcription-translation experiments confirm this size, and Northern (RNA) blot experiments indicatethat the protease is translated from a 33-kb mRNA. Searching the EMBLprotein database revealed that the amino acid sequence of the revisedPrtT is similar to sequences of two related proteins from Streptococcus pyogenes. PrtT is 31% identical and 73% similar over 401 amino acids to streptococcal pyrogenic exotoxin B. In addition, it is 36% identical and 74% similar over 244 amino acids with streptococcal proteinase, which is closely related to streptococcal pyrogenic exotoxin B. The similarity is particularly high at the putative active site of streptococcal proteinase, which is similar to the active sites of the family of cysteine proteases. Thus, we conclude that PrtT is a 96- to 99-kDa cysteine protease and hemagglutinin with significant similarity to streptococcal enzymes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:53:32