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Titolo:
2 DIFFERENT ENZYMES ARE PRIMARILY RESPONSIBLE FOR RETINOIC ACID SYNTHESIS IN RABBIT LIVER CYTOSOL
Autore:
HUANG DY; ICHIKAWA Y;
Indirizzi:
KAGAWA MED SCH,DEPT BIOCHEM MIKI KAGAWA 76107 JAPAN
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 2, volume: 205, anno: 1994,
pagine: 1278 - 1283
SICI:
0006-291X(1994)205:2<1278:2DEAPR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALDEHYDE DEHYDROGENASES; ENZYMATIC OXIDATION; MOUSE EPIDERMIS; RAT-TISSUES; INVITRO; IDENTIFICATION; RETINALDEHYDE; METABOLISM; CATALYZE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
D.Y. Huang e Y. Ichikawa, "2 DIFFERENT ENZYMES ARE PRIMARILY RESPONSIBLE FOR RETINOIC ACID SYNTHESIS IN RABBIT LIVER CYTOSOL", Biochemical and biophysical research communications, 205(2), 1994, pp. 1278-1283

Abstract

Retinoic acid biosynthesis in rabbit liver wets catalyzed by cytosolic NAD(+)-dependent dehydrogenase and oxygen-dependent oxidase, with;Inactivity ratio of 59% and 41% in the presence of 2 mM dithiothreitol under aerobic conditions. The two enzymes could be well separated by fractionation involving ammonium sulfate precipitation. Purification ofthe enzymes indicated that the oxygen-dependent enzyme was a flavoenzyme, retinal oxidase (EC 1.2.3.11), composed of two 135 kDa subunits; and the NAD(+)-dependent enzyme was a basic pI retinal dehydrogenase composed of four 55-kDa subunits. A high concentration (1-2 mM) of DTT was required to stabilize the activity of retinal dehydrogenase duringthe purification procedures and storage, but inhibited the activity of retinal oxidase by 13-38%. The physiological roles of the two retinoic acid synthases in liver cytosol were discussed. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:15:42