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Titolo:
THE T-ALPHA-1 ALPHA-TUBULIN PROMOTER SPECIFIES GENE-EXPRESSION AS A FUNCTION OF NEURONAL GROWTH AND REGENERATION IN TRANSGENIC MICE
Autore:
GLOSTER A; WU W; SPEELMAN A; WEISS S; CAUSING C; POZNIAK C; REYNOLDS B; CHANG E; TOMA JG; MILLER FD;
Indirizzi:
MONTREAL NEUROL HOSP & INST,CTR NEURONAL SURVIVAL,FIELDHOUSE LABS,3801 RUE UNIV MONTREAL PQ H3A 2B4 CANADA MONTREAL NEUROL HOSP & INST,CTR NEURONAL SURVIVAL,FIELDHOUSE LABS MONTREAL PQ H3A 2B4 CANADA MCGILL UNIV,MONTREAL NEUROL INST,DEPT NEUROL & NEUROSURG MONTREAL PQ H3A 2B4 CANADA UNIV CALGARY,HLTH SCI CTR,DEPT ANAT CALGARY AB T2N 4N1 CANADA UNIV ALBERTA,DEPT ANAT & CELL BIOL EDMONTON AB T6G 2H7 CANADA
Titolo Testata:
The Journal of neuroscience
fascicolo: 12, volume: 14, anno: 1994,
pagine: 7319 - 7330
SICI:
0270-6474(1994)14:12<7319:TTAPSG>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; TRANSCRIPTION FACTORS; NERVOUS-SYSTEM; CELLS; RAT; PERIPHERIN; INDUCTION; PROTEINS; ISOTYPES; ELEMENT;
Keywords:
NEURONAL DEVELOPMENT; NEURONAL REGENERATION; NEURONAL GROWTH; ALPHA-TUBULIN; NEURONAL PROMOTERS; TRANSGENIC MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
A. Gloster et al., "THE T-ALPHA-1 ALPHA-TUBULIN PROMOTER SPECIFIES GENE-EXPRESSION AS A FUNCTION OF NEURONAL GROWTH AND REGENERATION IN TRANSGENIC MICE", The Journal of neuroscience, 14(12), 1994, pp. 7319-7330

Abstract

We have previously demonstrated that one member of the alpha-tubulin multigene family, termed T alpha 1 in rats, is regulated as a functionof neuronal growth and regeneration. To elucidate the molecular mechanisms responsible for coupling gene expression to morphological differentiation, we have isolated the T alpha 1 gene, have fused 1.1 kb of the 5' flanking region to a nuclear lacZ reporter gene, and have generated transgenic mice. Analysis of these transgenic mice demonstrated that marker gene expression was specific to the CNS and PNS, with expression in vivo at embryonic day 13.5 being similar to expression of the endogenous gene. Moreover, the induction of transgene expression was correlated temporally with neuronal commitment in developing neural crest-derived peripheral neurons and in the developing retina. Immunocytochemical analysis of mixed primary embryonic brain cultures confirmed that transgene expression was specific to neurons, with the majority of neurons, but not astrocytes or oligodendrocytes, expressing beta-galactosidase. Transgene expression in vivo was maintained in developing neurons until early in postnatal life, subsequent to which its expression decreased coincident with neuronal maturation. The transgene was then reinduced in regenerating facial motoneurons following unilateral axotomy of the facial nerve. Thus, 1.1 kb of 5' flanking sequence fromthe T alpha 1 gene contains the sequence elements responsible for specifying gene expression to embryonic neurons and for subsequently regulating gene expression in both developing and mature neurons as a function of morphological growth.

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Documento generato il 19/09/20 alle ore 07:34:15