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Titolo:
STABLE MAINTENANCE OF AN INTEGRATED GENE IN NUCELLAR CELLS OF NAVEL ORANGE (CITRUS-SINENSIS OSB) UNDER STORAGE IN LN(2)
Autore:
KOBAYASHI S; SAKAI A; OHGAWARA T; NAKAMURA Y;
Indirizzi:
FRUIT TREE RES STN,AKITSU BRANCH AKITSU HIROSHIMA 72924 JAPAN KIKKOMAN FOODS INC,DIV RES & DEV NODA CHIBA 278 JAPAN
Titolo Testata:
Engei Gakkai Zasshi
fascicolo: 3, volume: 63, anno: 1994,
pagine: 553 - 558
SICI:
0013-7626(1994)63:3<553:SMOAIG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWN SHOOT TIPS; CRYOPRESERVATION; VITRIFICATION; PROTOPLASTS; TRANSFORMATION; REGENERATION; PLANTS; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
S. Kobayashi et al., "STABLE MAINTENANCE OF AN INTEGRATED GENE IN NUCELLAR CELLS OF NAVEL ORANGE (CITRUS-SINENSIS OSB) UNDER STORAGE IN LN(2)", Engei Gakkai Zasshi, 63(3), 1994, pp. 553-558

Abstract

Transformed nucellar cells of navel orange obtained by electroporation, were successfully cryopreserved in liquid nitrogen (LN(2)) by a vitrification method. The cells treated with a concentrated cryoprotective solution (PVS2) at 25 degrees C for 3 min were transferred to a 1.8 ml plastic cryotube and then directly plunged into LN(2). After storage for 1 year in LN(2), cell viability was about 90%, as evaluated by fluorescein diacetate (FDA) and phenosafranine double staining. Southern blot analysis showed that the integrated neomycin-phosphotransferaseII (NTPII) gene was stably maintained in the cryopreserved-regrown cells without DNA alteration. No morphological differences were observedbetween plants regenerated from cryopreserved cells and those from non-cryopreserved cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:07:17