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Titolo:
CELL-SPECIFIC EXPRESSION OF EPITHELIAL SODIUM-CHANNEL ALPHA-SUBUNITS,BETA-SUBUNITS, AND GAMMA-SUBUNITS IN ALDOSTERONE-RESPONSIVE EPITHELIAFROM THE RAT - LOCALIZATION BY IN-SITU HYBRIDIZATION AND IMMUNOCYTOCHEMISTRY
Autore:
DUE C; FARMAN N; CANESSA CM; BONVALET JP; ROSSIER BC;
Indirizzi:
UNIV LAUSANNE,INST PHARMACOL & TOXICOL,BUGNON 27 CH-1005 LAUSANNE SWITZERLAND UNIV LAUSANNE,INST PHARMACOL & TOXICOL CH-1005 LAUSANNE SWITZERLAND UNIV LAUSANNE,INST HISTOL & EMBRYOL CH-1005 LAUSANNE SWITZERLAND UER XAVIER BICHAT,INSERM,U246 F-75877 PARIS 18 FRANCE
Titolo Testata:
The Journal of cell biology
fascicolo: 6, volume: 127, anno: 1994,
parte:, 2
pagine: 1907 - 1921
SICI:
0021-9525(1994)127:6<1907:CEOESA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDULLARY COLLECTING DUCT; HUMAN SWEAT DUCT; CYSTIC-FIBROSIS; APICAL MEMBRANE; DISTAL COLON; ELECTROLYTE TRANSPORT; CATION CHANNEL; ION-TRANSPORT; NA CHANNELS; K+-ATPASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
C. Due et al., "CELL-SPECIFIC EXPRESSION OF EPITHELIAL SODIUM-CHANNEL ALPHA-SUBUNITS,BETA-SUBUNITS, AND GAMMA-SUBUNITS IN ALDOSTERONE-RESPONSIVE EPITHELIAFROM THE RAT - LOCALIZATION BY IN-SITU HYBRIDIZATION AND IMMUNOCYTOCHEMISTRY", The Journal of cell biology, 127(6), 1994, pp. 1907-1921

Abstract

A highly selective, amiloride-sensitive, epithelial sodium channel from rat colon (rENaC), composed of three homologous subunits termed alpha, beta, and gamma rENaC, has been cloned by functional expression and was proposed to mediate electrogenic sodium reabsorption in aldosterone-responsive epithelia. To determine whether rENaC could account forsodium absorption in vivo, we studied the cellular localization of the sodium channel messenger RNA subunits by in situ hybridization and their cellular and subcellular distribution by immunocytochemistry in the kidney, colon, salivary, and sweat glands of the rat. In the kidney, we show that the three subunit mRNAs are specifically co-expressed in the renal distal convoluted tubules (DCT), connecting tubules (CNT),cortical collecting ducts (CCD), and outer medullary collecting ducts(OMCD), but not in the inner medullary collecting ducts (IMCD). We demonstrate co-localization of alpha, beta, and gamma subunit proteins in the apical membrane of a majority of cells of CCD and OMCD. Our dataindicate that alpha, beta, and gamma subunit mRNAs and proteins are co-expressed in the distal nephron (excepting IMCD), a localization that correlates with the previously described physiological expression ofamiloride-sensitive electrogenic sodium transport. Our data, however,suggest that another sodium transport protein mediates electrogenic amiloride-sensitive sodium reabsorption in IMCD. We also localized rENaC to the surface epithelial cells of the distal colon and to the secretory ducts of the salivary gland and sweat gland, providing further evidence consistent with the hypothesis that the highly selective, amiloride-sensitive sodium channel is physiologically expressed in aldosterone-responsive cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 22:01:45