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Titolo:
DOWN-REGULATION OF G-ALPHA(S) IN HUMAN MYOMETRIUM IN TERM AND PRETERMLABOR - A MECHANISM FOR PARTURITION
Autore:
EUROPEFINNER GN; PHANEUF S; TOLKOVSKY AM; WATSON SP; BERNAL AL;
Indirizzi:
UNIV OXFORD,JOHN RADCLIFFE HOSP,DEPT OBSTET & GYNECOL OXFORD OX3 9DU ENGLAND UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT OBSTET & GYNECOL OXFORDOX3 9DU ENGLAND UNIV CAMBRIDGE,DEPT BIOCHEM CAMBRIDGE CB2 1QW ENGLAND UNIV OXFORD,DEPT PHARMACOL OXFORD OX1 3QT ENGLAND
Titolo Testata:
The Journal of clinical endocrinology and metabolism
fascicolo: 6, volume: 79, anno: 1994,
pagine: 1835 - 1839
SICI:
0021-972X(1994)79:6<1835:DOGIHM>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG MYOMETRIUM; ADENYLATE-CYCLASE; G-PROTEINS; PHOSPHOINOSITIDE HYDROLYSIS; PROSTAGLANDIN-E; ALPHA-SUBUNIT; PREGNANCY; ACTIVATION; RECEPTORS; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
G.N. Europefinner et al., "DOWN-REGULATION OF G-ALPHA(S) IN HUMAN MYOMETRIUM IN TERM AND PRETERMLABOR - A MECHANISM FOR PARTURITION", The Journal of clinical endocrinology and metabolism, 79(6), 1994, pp. 1835-1839

Abstract

We have previously reported that G alpha(s) is expressed at considerably higher levels in myometrium taken from pregnant than from nonpregnant women. In the present study we have determined adenylyl cyclase activity in myometrial membranes by measuring the conversion of [alpha-P-32 to [P-32]cAMP and have measured guanosine triphosphate-binding protein expression by immunoblotting with specific antibodies. Here we report that the increase in G alpha(s) expression in pregnant myometriumis associated with a significant increase in G alpha(s)-coupled adenylyl cyclase activity, as estimated by incubating myometrial membranes in the presence of 5'-guanylyl-imidodiphosphate with and without prostaglandin E(2). Moreover, in myometrium from women in spontaneous laborG alpha(s) levels and G alpha(s)-coupled adenylyl cyclase activity are reduced to the levels observed in nonpregnant tissue. There was no apparent change in forskolin-stimulated adenylyl cyclase activity in nonpregnant, pregnant, and laboring tissue. The increase in G alpha(s) expression in pregnant myometrium may facilitate agonist-induced cAMP formation, resulting in prolonged relaxation of the uterus during gestation. Down-regulation of G alpha(s) would decrease the relaxing effectexerted by cAMP and may be a triggering mechanism for the initiation of labor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:08:38