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Titolo:
STRUCTURALLY RELATED BUT FUNCTIONALLY DISTINCT YEAST SM-D CORE SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE PROTEINS
Autore:
ROY J; ZHENG BH; RYMOND BC; WOOLFORD JL;
Indirizzi:
CARNEGIE MELLON UNIV,DEPT BIOL SCI,4400 5TH AVE PITTSBURGH PA 15213 CARNEGIE MELLON UNIV,DEPT BIOL SCI PITTSBURGH PA 15213 UNIV KENTUCKY,TH MORGAN SCH BIOL SCI LEXINGTON KY 40506
Titolo Testata:
Molecular and cellular biology
fascicolo: 1, volume: 15, anno: 1995,
pagine: 445 - 455
SICI:
0270-7306(1995)15:1<445:SRBFDY>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
60S RIBOSOMAL-SUBUNITS; L929 MOUSE FIBROBLASTS; SACCHAROMYCES-CEREVISIAE; SPLICING INVITRO; SNRNP PARTICLES; XENOPUS-LAEVIS; RNA; BINDING; U1; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
J. Roy et al., "STRUCTURALLY RELATED BUT FUNCTIONALLY DISTINCT YEAST SM-D CORE SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE PROTEINS", Molecular and cellular biology, 15(1), 1995, pp. 445-455

Abstract

Spliceosome assembly during pre-mRNA splicing requires the correct positioning of the U1, U2, U4/U6, and U5 small nuclear ribonucleoproteinparticles (snRNPs) on the precursor mRNA. The structure and integrityof these snRNPs are maintained in part by the association of the snRNAs with core snRNP (Sm) proteins. The Sm proteins also play a pivotal role in metazoan snRNP biogenesis. We have characterized a Saccharomyces cerevisiae gene, SMD3, that encodes the core snRNP protein Smd3. The Smd3 protein is required for pre-mRNA splicing in vivo. Depletion ofthis protein from yeast cells affects the levels of U snRNAs and their cap modification, indicating that Smd3 is required for snRNP biogenesis. Smd3 is structurally and functionally distinct from the previously described yeast core polypeptide Smd1. Although Smd3 and Smd1 are both associated with the spliceosomal snRNPs, overexpression of one cannot compensate for the loss of the other. Thus, these two proteins havedistinct functions. A pool of Smd3 exists in the yeast cytoplasm. This is consistent with the possibility that snRNP assembly in S. cerevisiae, as in metazoans, is initiated in the cytoplasm from a pool of RNA-free core snRNP protein complexes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:31:48