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Titolo:
CHARACTERIZATION OF DNA-SYNTHESIS AND DNA-DEPENDENT ATPASE ACTIVITY AT A RESTRICTIVE TEMPERATURE IN TEMPERATURE-SENSITIVE TSFT848 CELLS WITH THERMOLABILE DNA HELICASE-B
Autore:
SEKI M; KOHDA T; YANO T; TADA S; YANAGISAWA J; EKI T; UI M; ENOMOTO T;
Indirizzi:
UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PHYSIOL CHEM,BUNKYO KU,7-3-1 HONGOTOKYO 113 JAPAN UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PHYSIOL CHEM,BUNKYO KU TOKYO 113 JAPAN INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,DIV HUMAN GENOME RES TSUKUBA IBARAKI 305 JAPAN INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,GENE BANK TSUKUBA IBARAKI 305 JAPAN
Titolo Testata:
Molecular and cellular biology
fascicolo: 1, volume: 15, anno: 1995,
pagine: 165 - 172
SICI:
0270-7306(1995)15:1<165:CODADA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE FM3A CELLS; SINGLE-STRANDED-DNA; NUCLEOTIDE EXCISION REPAIR; SACCHAROMYCES-CEREVISIAE; XERODERMA-PIGMENTOSUM; CALF THYMUS; ADENOSINETRIPHOSPHATASE-B; COMPLEMENTATION GROUP; BINDING-PROTEIN; SV40 ORIGIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
M. Seki et al., "CHARACTERIZATION OF DNA-SYNTHESIS AND DNA-DEPENDENT ATPASE ACTIVITY AT A RESTRICTIVE TEMPERATURE IN TEMPERATURE-SENSITIVE TSFT848 CELLS WITH THERMOLABILE DNA HELICASE-B", Molecular and cellular biology, 15(1), 1995, pp. 165-172

Abstract

A temperature-sensitive mutant defective in DNA replication, tsFT848,was isolated from the mouse mammary carcinoma cell line FM3A. In mutant cells, the DNA-dependent ATPase activity of DNA helicase B, which is a major DNA-dependent ATPase in wild-type cells, decreased at the nonpermissive temperature of 39 degrees C. DNA synthesis in tsFT848 cells at the nonpermissive temperature was analyzed in detail. DNA synthesis measured by incorporation of [H-3]thymidine decreased to about 50% and less than 10% of the initial level at 8 and 12 h, respectively. The decrease in the level of thymidine incorporation correlated,vith a decrease in the number of silver grains in individual nuclei but not with the number of cells with labeled nuclei. DNA fiber autoradiography revealed that the DNA chain elongation rate did not decrease even after an incubation for 10 h at 39 degrees C, suggesting that initiation of DNA replication at the origin of replicons is impaired in the mutantcells. The decrease in DNA-synthesizing ability coincided with a decrease in the level of the DNA-dependent ATPase activity of DNA helicaseB. Partially purified DNA helicase B from tsFT848 cells was more heatsensitive than that from wild-type cells. Inactivation of DNA-dependent ATPase activity of DNA helicase B from mutant cells was considerably reduced by adding DNA to the medium used for preincubation, indicating that the DNA helicase of mutant cells is stabilized by binding to DNA.

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Documento generato il 23/09/20 alle ore 05:35:12