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Titolo:
MOLECULAR-CLONING AND FUNCTIONAL-CHARACTERIZATION OF THE OXYTOCIN RECEPTOR FROM A RAT PANCREATIC-CELL LINE (RINM5F)
Autore:
JENG YJ; LOLAIT SJ; STRAKOVA Z; CHEN C; COPLAND JA; MELLMAN D; HELLMICH MR; SOLOFF MS;
Indirizzi:
UNIV TEXAS,MED BRANCH,DEPT OBSTET & GYNECOL,301 UNIV BLVD GALVESTON TX 77555 UNIV TEXAS,MED BRANCH,DEPT OBSTET & GYNECOL GALVESTON TX 77555 UNIV TEXAS,MED BRANCH,DEPT SURG GALVESTON TX 77555 UNIV TEXAS,MED BRANCH,SEALY CTR MOL SCI GALVESTON TX 77555 NIMH,LAB CELLULAR & MOL REGULAT BETHESDA MD 20892
Titolo Testata:
Neuropeptides
fascicolo: 6, volume: 30, anno: 1996,
pagine: 557 - 565
SICI:
0143-4179(1996)30:6<557:MAFOTO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; MAMMARY-GLAND; ARGININE-VASOPRESSIN; SIGNAL-TRANSDUCTION; OVINE ENDOMETRIUM; INSULIN RELEASE; NORMAL MOUSE; FREE CALCIUM; EXPRESSION; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
Y.J. Jeng et al., "MOLECULAR-CLONING AND FUNCTIONAL-CHARACTERIZATION OF THE OXYTOCIN RECEPTOR FROM A RAT PANCREATIC-CELL LINE (RINM5F)", Neuropeptides, 30(6), 1996, pp. 557-565

Abstract

Oxytocin (OT) and vasopressin (AVP) stimulate insulin and glucagon release from the pancreas, and evoke insulin secretion from the rat insulinoma cell line, RINm5F. To determine which AVP/OT receptor subtype is expressed in RINm5F cells, we used PCR with degenerate primers to two transmembrane domains of the AVP (Via, V1b (or V3), V2) and OT receptors (OTRs). The single PCR fragment identified was used to obtain a full length cDNA from a RINm5F cDNA library. Comparison of the deduced amino acid sequence of this clone with uterine OTR sequences from several species (human, sheep, bovine) and to the pig kidney epithelial cell (LLC-PK1) OTR reveals a very high degree of homology. After the RINcell OTR cDNA was stably transfected into CHO cells (CHO-OTR), the cell membranes bound iodinated oxytocin antagonist with an apparent K-d comparable to that of RIN cell membranes and those from other OT target cells. Comparison of the ligand specificities of CHO-OTR and RIN cells membranes showed that the relative K-i values of a series of OT analogues were approximately equivalent in both preparations. The rank order of apparent K-i values also corresponded to published values for the rat myometrium, where OT elicits intracellular calcium transients, and increases inositol phosphate production. In uterine endometrium and amnion cells, OT stimulates prostaglandin release. Stimulation of CHO-OTR cells with OT caused an increase in cytosolic calcium concentration originating from both intracellular and extracellular sources, anda dose-dependent increase in inositol phosphate levels. Arachidonic acid release and PGE, synthesis were also stimulated by OT These findings (amino acid sequence homology, binding specificity, and signal transduction/second messenger production) suggest that OTRs from RINm5F cells are indistinguishable from OTRs that have been described in other tissues. The expression of OTR in pancreatic cells implies that OT plays a role in pancreatic function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 05:53:26