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Titolo:
LOCATION OF THE PH-20 PROTEIN ON ACROSOME-INTACT AND ACROSOME-REACTEDSPERMATOZOA OF CYNOMOLGUS MACAQUES
Autore:
OVERSTREET JW; LIN Y; YUDIN AI; MEYERS SA; PRIMAKOFF P; MYLES DG; KATZ DF; VANDEVOORT CA;
Indirizzi:
UNIV CALIF DAVIS,CALIF REG PRIMATE RES CTR,SUBER HOUSE DAVIS CA 95616 UNIV CALIF DAVIS,SCH MED,DEPT OBSTET & GYNECOL,DIV REPROD BIOL & MED DAVIS CA 95616 UNIV CONNECTICUT,CTR HLTH,DEPT PHYSIOL FARMINGTON CT 06030
Titolo Testata:
Biology of reproduction
fascicolo: 1, volume: 52, anno: 1995,
pagine: 105 - 114
SICI:
0006-3363(1995)52:1<105:LOTPPO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG SPERM; EGG ZONA PELLUCIDA; MOUSE GAMETE INTERACTIONS; SURFACE GALACTOSYLTRANSFERASE; FERTILIZATION INVITRO; MAMMALIAN SPERM; BINDING; RECEPTOR; CHLORTETRACYCLINE; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J.W. Overstreet et al., "LOCATION OF THE PH-20 PROTEIN ON ACROSOME-INTACT AND ACROSOME-REACTEDSPERMATOZOA OF CYNOMOLGUS MACAQUES", Biology of reproduction, 52(1), 1995, pp. 105-114

Abstract

Fluorescence microscopy and transmission electron microscopy (TEM) were used to determine the location of the membrane protein PH-20 on spermatozoa of cynomolgus macaques. Rabbit antiserum raised against recombinant cynomolgus macaque sperm PH-20 was used as the primary antibody, and the second antibody was goat anti-rabbit IgG conjugated with either fluorescein isothiocyanate or 15 nm gold particles. Spermatozoa were evaluated before capacitation and after capacitation and induction of acrosome reactions with calcium ionophore A23187. In sperm suspensions with a high percentage of intact acrosomes, fluorescence labeling was observed uniformly over most of the sperm head. The sperm midpieceand tail were not labeled. In sperm suspensions with a high percentage of acrosome reactions, most spermatozoa labeled intensely over the anterior sperm head, but labeling of the posterior sperm head was greatly reduced. TEM of acrosome-intact spermatozoa revealed gold particlesdistributed uniformly on the plasma membrane overlying the acrosome, the equatorial segment, and mot of the post-acrosomal region. After the acrosome reaction, gold label was present on the inner acrosomal membrane and on the plasma membrane overlying the equatorial segment. Very little label was present on the plasma membrane in the post-acrosomal region of acrosome-reacted spermatozoa. The location of PH-20 on thesurface of macaque spermatozoa suggests a function for this protein in primary and/or secondary binding to the zona pellucida. The apparentdecrease in amount of PH-20 on the posterior head of macaque spermatozoa following the acrosome reaction is consistent with the migration of this protein to the inner acrosomal membrane, as demonstrated previously for the homologous PH-20 protein of guinea pig spermatozoa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 13:34:05