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Titolo:
PROPERTIES OF SILIBININ AND OF ANTIOXIDANTS AGAINST ADRIAMYCIN CYTOTOXICITY IN A UNICELLULAR EUKARYOTE, TETRAHYMENA-THERMOPHILA
Autore:
SOOSE M;
Indirizzi:
UNIV GIESSEN,INST TIERPHYSIOL,WARTWEG 95 D-35392 GIESSEN GERMANY
Titolo Testata:
European journal of protistology
fascicolo: 4, volume: 30, anno: 1994,
pagine: 394 - 403
SICI:
0932-4739(1994)30:4<394:POSAOA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED LIPID-PEROXIDATION; CHEMOTHERAPEUTIC-AGENTS; DOXORUBICIN ADRIAMYCIN; CELLULAR UPTAKE; FREE-RADICALS; HEAVY-METALS; GLUTATHIONE; DNA; SILYMARIN; CELLS;
Keywords:
ADRIAMYCIN; CYTOPROTECTION; SILIBININ; ANTIOXIDANTS; TETRAHYMENA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
M. Soose, "PROPERTIES OF SILIBININ AND OF ANTIOXIDANTS AGAINST ADRIAMYCIN CYTOTOXICITY IN A UNICELLULAR EUKARYOTE, TETRAHYMENA-THERMOPHILA", European journal of protistology, 30(4), 1994, pp. 394-403

Abstract

To elucidate the primary mechanism of adriamycin (ADR) cytotoxicity the properties of the flavonoid silibinin and of antioxidants against the cellular response to the anthracyclin drug were compared in Tetrahymena thermophila. Exponentially growing cultures were preincubated for30 min with either silibinin, butylated hydroxyanisole (BHA) or a ''cocktail'' of superoxide dismutase (SOD), catalase and N-acetylcysteine(NAC), and then continuously exposed to these substances together with ADR (10 and 20 mu g/ml). ADR administration alone resulted in a celldivision arrest which was correlated with an inhibition of RNA synthesis and a minor and delayed reduction of protein synthesis. The inhibitory effects of ADR on growth and biomass production were completely prevented by silibinin (1 mg/ml). ADR uptake into silibinin-treated cells was proved by fluorescence microscopy. Antioxidants had no effect on ADR cytotoxicity. It is therefore suggested that redox processes arenot the primary mechanism of ADR cytotoxicity in rapidly dividing cells and that cytoprotection by silibinin in this system is not due to its antioxidant properties. The data are rather in favour of ADR-DNA interactions and of a direct stimulatory effect of silibinin on RNA synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:40:12