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Titolo:
INDUCTION OF VASOACTIVE-INTESTINAL-PEPTIDE GENE-EXPRESSION AND PROLACTIN SECRETION BY INSULIN-LIKE GROWTH-FACTOR-I IN RAT PITUITARY-CELLS -EVIDENCE FOR AN AUTOPARACRINE REGULATORY SYSTEM
Autore:
LARA JI; LORENZO MJ; CACICEDO L; TOLON RM; BALSA JA; LOPEZFERNANDEZ J; SANCHEZFRANCO F;
Indirizzi:
INST SALUD CARLOS 3,CNIC,SERV ENDOCRINOL,SINESIO DELGADO 10-12 E-28029 MADRID SPAIN INST SALUD CARLOS 3,CNIC,SERV ENDOCRINOL E-28029 MADRID SPAIN HOSP RAMON Y CAJAL,SERV ENDOCRINOL E-28034 MADRID SPAIN FDN JIMENEZ DIAZ,SERV ENDOCRINOL E-28034 MADRID SPAIN
Titolo Testata:
Endocrinology
fascicolo: 6, volume: 135, anno: 1994,
pagine: 2526 - 2532
SICI:
0013-7227(1994)135:6<2526:IOVGAP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE NEGATIVE FEEDBACK; ANTERIOR-PITUITARY; MESSENGER-RNA; POLYPEPTIDE; CULTURE; RELEASE; TISSUE; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
J.I. Lara et al., "INDUCTION OF VASOACTIVE-INTESTINAL-PEPTIDE GENE-EXPRESSION AND PROLACTIN SECRETION BY INSULIN-LIKE GROWTH-FACTOR-I IN RAT PITUITARY-CELLS -EVIDENCE FOR AN AUTOPARACRINE REGULATORY SYSTEM", Endocrinology, 135(6), 1994, pp. 2526-2532

Abstract

The effects of recombinant human insulin-like growth factor I (IGF-I)on both vasoactive intestinal peptide (VIP) and PRL production and gene expression were studied using rat anterior pituitary cell, culturesgrown in serum-free defined medium. We also examined whether pituitary VIP could be involved in the PRL response to IGF-I and hence in a paracrine regulatory system. Exposure of cultured anterior pituitary cells to IGF-I (2.6 nM) for 3 h caused a significant decrease in both VIPcontent and media PRL. Treatment with IGF-I (from 0.65-5.2 nM) for 48h increased VIP production and VIP messenger RNA (mRNA) accumulation,whereas only an increase in media and intracellular PRL content without changes in mRNA was observed. In all these experiments, IGF-I led to a decrease in both GH secretion and expression. Immunoglobulins G purified from VIP antiserum inhibited IGF-I-induced PRL release without affecting intracellular and mRNA levels. The inhibition of both GH secretion and gene expression induced by IGF-I was not blocked by VIP antiserum. In conclusion, these results indicate that IGF-I induces VIP gene expression, and its secretion and also increases PRL secretion. The effect of IGF-I on PRL release is specifically mediated by VIP through a paracrine or autocrine mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:24:10