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Titolo:
THE PCA-POB SUPRAOPERONIC CLUSTER OF ACINETOBACTER-CALCOACETICUS CONTAINS QUIA, THE STRUCTURAL GENE FOR QUINATE-SHIKIMATE DEHYDROGENASE
Autore:
ELSEMORE DA; ORNSTON LN;
Indirizzi:
YALE UNIV,DEPT BIOL,POB 6666 NEW HAVEN CT 06520 YALE UNIV,DEPT BIOL NEW HAVEN CT 06520
Titolo Testata:
Journal of bacteriology
fascicolo: 24, volume: 176, anno: 1994,
pagine: 7659 - 7666
SICI:
0021-9193(1994)176:24<7659:TPSCOA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
P-HYDROXYBENZOATE HYDROXYLASE; GRAM-NEGATIVE BACTERIA; EVOLUTIONARY DIVERGENCE; RHODOBACTER-CAPSULATUS; PSEUDOMONAS-PUTIDA; ORGANIZATION; CLONING; DNA; PROTOCATECHUATE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
D.A. Elsemore e L.N. Ornston, "THE PCA-POB SUPRAOPERONIC CLUSTER OF ACINETOBACTER-CALCOACETICUS CONTAINS QUIA, THE STRUCTURAL GENE FOR QUINATE-SHIKIMATE DEHYDROGENASE", Journal of bacteriology, 176(24), 1994, pp. 7659-7666

Abstract

An 18-kbp Acinetobacter calcoaceticus chromosomal segment contains the pcaIJFBDKCHG operon, which is required for catabolism of protocatechuate, and pobSRA, genes associated with conversion of p-hydroxybenzoate to protocatechuate. The genetic function of the 6.5 kbp of DNA between pcaG and pobS was unknown. Deletions in this DNA mere designed by removal of fragments between restriction sites, and the deletion mutations were introduced into A. calcoaceticus by natural transformation. The mutations prevented growth with either quinate or shikimate, growthsubstrates that depend upon qui gene function for their catabolism toprotocatechuate. The location of quiA, a gene encoding quinate-shikimate dehydrogenase, was indicated by its expression in one of the deletion mutants, and the position of the gene was confirmed by determination of its 2,427-bp nucleotide sequence. The deduced amino acid sequence of QuiA confirmed that it is a member of a family of membrane-associated, pyrrolo-quinoline quinone-dependent dehydrogenases, as had been suggested by earlier biochemical investigations. Catabolism of quinateand shikimate is initiated by NAD(+)-dependent dehydrogenases in other microorganisms, so it is evident that different gene pools were called upon to provide the ancestral enzyme for this metabolic step.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:32:00