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Titolo:
CHAPERONE-MEDIATED ACTIVATION IN-VIVO OF A PSEUDOMONAS-CEPACIA LIPASE
Autore:
AAMAND JL; HOBSON AH; BUCKLEY CM; JORGENSEN ST; DIDERICHSEN B; MCCONNELL DJ;
Indirizzi:
UNIV DUBLIN TRINITY COLL,DEPT GENET DUBLIN 2 IRELAND UNIV DUBLIN TRINITY COLL,DEPT GENET DUBLIN 2 IRELAND UNIV DUBLIN TRINITY COLL,NATL PHARMACEUT BIOTECHNOL CTR DUBLIN 2 IRELAND NOVO NORDISK AS,DEPT BACTERIAL GENE TECHNOL DK-2880 BAGSVAERD DENMARK
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 5, volume: 245, anno: 1994,
pagine: 556 - 564
SICI:
0026-8925(1994)245:5<556:CAIOAP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; BACILLUS-SUBTILIS; MOLECULAR-CLONING; PROTEIN; GENE; EXPRESSION; AERUGINOSA; GROEL; DNA; CONSTRUCTION;
Keywords:
CHAPERONE; PSEUDOMONAS CEPACIA LIPASE; LIMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Aamand et al., "CHAPERONE-MEDIATED ACTIVATION IN-VIVO OF A PSEUDOMONAS-CEPACIA LIPASE", MGG. Molecular & general genetics, 245(5), 1994, pp. 556-564

Abstract

An extracellular Pseudomonas cepacia lipase, LipA, is inactive when expressed in the absence of the product of the limA gene. Evidence has been presented that LimA is a molecular chaperone. The lipA and limA genes have been cloned in separate and independently inducible expression systems in Escherichia coli. These systems were used to test the molecular chaperone hypothesis by investigating whether LimA could activate presynthesized prelipase and whether presynthesized LimA could activate newly synthesized prelipase. The results show that LimA cannot activate presynthesized prelipase and that presynthesized LimA can activate only a limited number of de novo synthesized prelipase molecules. Co-immunoprecipitation of prelipase/lipase with LimA generated a 1:1 complex of prelipase/lipase and LimA. The results suggest that a 1:1 complex of LipA and LimA is required for prelipase processing and secretion of active lipase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:30:03