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Titolo:
DETECTION OF IMMUNOLOGICALLY RELATED KUNITZ AND BOWMAN-BIRK PROTEINASE-INHIBITORS EXPRESSED DURING POTATO-TUBER DEVELOPMENT
Autore:
MITSUMORI C; YAMAGISHI K; FUJINO K; KIKUTA Y;
Indirizzi:
HOKKAIDO UNIV,FAC AGR,DEPT BOT SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO UNIV,FAC AGR,DEPT BOT SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Plant molecular biology
fascicolo: 3, volume: 26, anno: 1994,
pagine: 961 - 969
SICI:
0167-4412(1994)26:3<961:DOIRKA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOYBEAN TRYPSIN-INHIBITORS; TRANSGENIC TOBACCO PLANTS; INDUCIBLE GENE-EXPRESSION; MOLECULAR-CLONING; II PROMOTER; SEQUENCE; INVITRO; PATATIN; ELEMENT; LEAVES;
Keywords:
GENE EXPRESSION; KUNITZ-TYPE PROTEINASE INHIBITOR; POTATO (SOLANUM TUBEROSUM L); SOYBEAN C-II INHIBITOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
C. Mitsumori et al., "DETECTION OF IMMUNOLOGICALLY RELATED KUNITZ AND BOWMAN-BIRK PROTEINASE-INHIBITORS EXPRESSED DURING POTATO-TUBER DEVELOPMENT", Plant molecular biology, 26(3), 1994, pp. 961-969

Abstract

Antiserum against a potato Kunitz-type proteinase inhibitor (PKPI) expressed in Escherichia coli was produced. In immunoblotting assays of proteins from potato tubers cultured in vitro, three proteins reacted to the antiserum, two of 20 kDa and one of 10 kDa. Their N-termini were sequenced. While the 20 kDa proteins showed 59 and 90% identity to PKPI, the 10 kDa one had 65% identity to soybean C-II proteinase inhibitor. Characterization of the temporal expression of these proteins showed that both could be detected from 10 days after induction of tuberization (DAI) in vitro, but the times when maximum amounts of PKPI and 10 kDa protein could be detected were different, corresponding to 22 and 32 DAI, respectively. The amounts of these proteins decreased in the following stages, and no positive reaction of the antiserum with mature tuber proteins could be found. The 20 kDa proteins were also detected in early stages of development of potato tubers grown in the held,indicating that these proteins are expressed during normal tuber development, and differ from the PKPIs reported previously.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:15:38