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Titolo:
ADAPTATION TO PERSISTENT GROWTH IN THE H9 CELL-LINE RENDERS A PRIMARYISOLATE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SENSITIVE TO NEUTRALIZATION BY VACCINE SERA
Autore:
WRIN T; LOH TP; VENNARI JC; SCHUITEMAKER H; NUNBERG JH;
Indirizzi:
137 ALBERTA AVE SAN CARLOS CA 94070 GENENTECH INC,RES VIROL LAB,DEPT IMMUNOL S SAN FRANCISCO CA 94080 NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,DEPT CLIN VIROIMMUNOL 1066 CX AMSTERDAM NETHERLANDS
Titolo Testata:
Journal of virology
fascicolo: 1, volume: 69, anno: 1995,
pagine: 39 - 48
SICI:
0022-538X(1995)69:1<39:ATPGIT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT SOLUBLE CD4; HUMAN MONOCLONAL-ANTIBODIES; AIDS-RELATED COMPLEX; V3 LOOP; SURFACE GLYCOPROTEIN; HIV-1 INFECTION; VIRAL ENVELOPE; GP120; DOMAIN; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
T. Wrin et al., "ADAPTATION TO PERSISTENT GROWTH IN THE H9 CELL-LINE RENDERS A PRIMARYISOLATE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 SENSITIVE TO NEUTRALIZATION BY VACCINE SERA", Journal of virology, 69(1), 1995, pp. 39-48

Abstract

Seven diverse primary isolates of human immunodeficiency virus type 1(HIV-1) mere examined and found to be refractory to neutralization byantisera to recombinant gp120 (rgp120) protein from HIV-1 MN. This stands in marked contrast to the sensitivity exhibited by certain laboratory-adapted viruses. To understand the difference between primary andlaboratory-adapted viruses, we adapted the primary virus ACH 168.10 to growth in the FDA/H9 cell line. ACH 168.10 was chosen because the V3region of gp120 closely matches that of MN. After 4 weeks, infection became evident. The virus (168A) replicated in FDA/H9 cells with extensive cytopathic effect but was unchanged in sensitivity to antibody-mediated neutralization. Thus, growth in cell lines is not sufficient torender primary virus sensitive to neutralization. The 168A virus was,however, partially sensitive to CD4 immunoadhesin (CD4-Ig). Adaptation was continued to produce a persistently infected FDA/H9 culture thatdisplayed minimal cytopathic effect. The virus (168C) was now sensitive to neutralization by MN rgp120 vaccine sera and by MN-specific monoclonal antibodies and showed increased sensitivity to HIVIG and CD4-Ig. 168C encoded three amino acid changes in gp120), including one within the V3 loop (I-166-->R, I-282-->N, G-318-->R). MN-specific monoclonal antibodies bound equally lo the surface of cells infected with either neutralization-resistant or -sensitive virus. The coincidence of changes in neutralization sensitivity with changes in cell tropism and cytopathic effect suggests a common underlying mechanism(s) acting through the whole of the envelope protein complex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:32:54