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Titolo:
SPERM FROM MICE CARRYING A TARGETED MUTATION OF THE ACROSIN GENE CAN PENETRATE THE OOCYTE ZONA-PELLUCIDA AND EFFECT FERTILIZATION
Autore:
BABA T; AZUMA S; KASHIWABARA S; TOYODA Y;
Indirizzi:
UNIV TSUKUBA,INST APPL BIOCHEM TSUKUBA IBARAKI 305 JAPAN UNIV TOKYO,INST MED SCI,DEPT REPROD & DEV BIOL,MINATO KU TOKYO 108 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 50, volume: 269, anno: 1994,
pagine: 31845 - 31849
SICI:
0021-9258(1994)269:50<31845:SFMCAT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRYPSIN-LIKE ACTIVITY; CDNA SEQUENCE; BOAR SPERMATOZOA; BINDING-PROTEIN; PROACROSIN; PURIFICATION; ACTIVATION; EXPRESSION; SPERMATOGENESIS; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T. Baba et al., "SPERM FROM MICE CARRYING A TARGETED MUTATION OF THE ACROSIN GENE CAN PENETRATE THE OOCYTE ZONA-PELLUCIDA AND EFFECT FERTILIZATION", The Journal of biological chemistry, 269(50), 1994, pp. 31845-31849

Abstract

The physiological function of mammalian sperm acrosin has long been believed to be involved in the limited proteolysis of the oocyte zona pellucida, thus enabling the sperm to penetrate this extracellular matrix and to gain access to the oocyte plasma membrane. Here we show thatmale mice homozygous for a targeted mutation in the mouse acrosin gene are still fertile in spite of the complete absence of acrosin protease activity in the sperm. In vitro fertilization assays verified that sperm from the homozygous mutant mice penetrate the zona pellucida andeffect fertilization. Therefore, acrosin is not essential for both sperm penetration of the zona pellucida and fertilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:40:39