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Titolo:
DEVELOPMENT OF AN ANTI-A-BETA MONOCLONAL-ANTIBODY FOR IN-VIVO IMAGINGOF AMYLOID ANGIOPATHY IN ALZHEIMERS-DISEASE
Autore:
FRIEDLAND RP; MAJOCHA RE; RENO JM; LYLE LR; MAROTTA CA;
Indirizzi:
CASE WESTERN RESERVE UNIV,DEPT NEUROL,2074 ABINGTON RD CLEVELAND OH 44106 MASSACHUSETTS GEN HOSP,NEUROBIOL LAB BOSTON MA 00000 BROWN UNIV,DEPT PSYCHIAT PROVIDENCE RI 00000 MALLINCKRODT MED INC ST LOUIS MO 00000 NEORX CORP SEATTLE WA 00000
Titolo Testata:
Molecular neurobiology
fascicolo: 1-3, volume: 9, anno: 1994,
pagine: 107 - 113
SICI:
0893-7648(1994)9:1-3<107:DOAAMF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLAQUE CORE PROTEIN; CONGOPHILIC ANGIOPATHY; PRECURSOR; DIAGNOSIS; THERAPY; INVIVO; CORTEX; BRAIN;
Keywords:
ALZHEIMERS DISEASE; AMYLOID ANGIOPATHY; IN VIVO DIAGNOSIS; MONOCLONAL ANTIBODIES; SINGLE PHOTON EMISSION TOMOGRAPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
R.P. Friedland et al., "DEVELOPMENT OF AN ANTI-A-BETA MONOCLONAL-ANTIBODY FOR IN-VIVO IMAGINGOF AMYLOID ANGIOPATHY IN ALZHEIMERS-DISEASE", Molecular neurobiology, 9(1-3), 1994, pp. 107-113

Abstract

We evaluated the efficacy of murine monoclonal antibodies (MAbs) targeted to the A beta amyloid of Alzheimer's disease for development of procedures for the in vivo identification of amyloid angiopathy (AA). MAbs to A beta were prepared and screened for effectiveness in visualizing AA and neuritic plaques in postmortem AD brain sections. They wereassessed again after enzymatic cleavage to produce Fab fragments and after labeling with technetium-99m (Tc-99m) using a diamide dimercaptide ligand system. Modified and radiolabeled Fab fragments retained activity and specificity toward amyloid-laden blood vessels and neuritic plaques. A highly specific murine MAb, 10H3, was identified and characterized that fulfills criteria necessary for the development of an in vivo diagnostic imaging agent. Toxicity studies in rats showed the MAbto be safe. Biodistribution studies in mice demonstrated desirable properties for use as an imaging agent. Expansion and adaptation of these strategies may provide the methods and materials for the noninvasiveanalysis of AA in living patients, and permit assessment of the contribution of AA to the clinical and pathological features of AD.

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Documento generato il 02/12/20 alle ore 05:11:33