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Titolo:
ANTI-HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ACTIVITY OF PHOSPHOROTHIOATEANALOGS OF OLIGODEOXYNUCLEOTIDES - PENETRATION AND LOCALIZATION OF OLIGODEOXYNUCLEOTIDES IN HIV-1-INFECTED MOLT4 CELLS
Autore:
NAKASHIMA H; SHOJI Y; KIM SG; SHIMADA J; MIZUSHIMA Y; ITO M; YAMAMOTO N; TAKAKU H;
Indirizzi:
YAMANASHI MED UNIV,DEPT MICROBIOL YAMANASHI 40938 JAPAN ST MARIANNA UNIV,SCH MED,INST MED SCI KAWASAKI KANAGAWA 216 JAPAN CHIBA INST TECHNOL,DEPT IND CHEM NARASHINO CHIBA 275 JAPAN TOKYO MED & DENT UNIV,SCH MED,DEPT MICROBIOL,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
Nucleic acids research
fascicolo: 23, volume: 22, anno: 1994,
pagine: 5004 - 5010
SICI:
0305-1048(1994)22:23<5004:ATAOP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONICALLY INFECTED-CELLS; ANTISENSE OLIGODEOXYNUCLEOTIDES; VIRAL-RNA; INHIBITION; REPLICATION; OLIGODEOXYRIBONUCLEOTIDE; EXPRESSION; AGENTS; OLIGONUCLEOTIDES; COMPLEMENTARY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
H. Nakashima et al., "ANTI-HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ACTIVITY OF PHOSPHOROTHIOATEANALOGS OF OLIGODEOXYNUCLEOTIDES - PENETRATION AND LOCALIZATION OF OLIGODEOXYNUCLEOTIDES IN HIV-1-INFECTED MOLT4 CELLS", Nucleic acids research, 22(23), 1994, pp. 5004-5010

Abstract

Phosphorothioate antisense oligodeoxynucleotide against HIV-1 rev (S-ODN-rev) inhibits virus-induced cytopathic effects (CPE) in acute infection and inhibits the expression of HIV-1 core protein, p24, in chronically infected cells in vitro. HIV-1 reverse transcriptase activity was not affected by S-ODN-rev at the high concentrations of 5-25 mu M, which were 250-1250 times higher than the concentration required to achieve 100% HIV-l-induced CPE inhibition. [P-32]-labeled S-ODN-rev was rapidly uptaken by MOLT-4 cells, whereas [P-32]-SO-ODN-rev and [P-32]-O-ODN-rev were not. In the observation of FITC-S-ODN-rev-treated MOLT-4 cells by a confocal laser scanning microscope, diffuse fluorescence was apparently observed in the cytoplasm. Interestingly, fluorescence signals were accumulated in the nuclear region of chronically infectedMOLT-4/HIV-1 cells 60 min after incubation. FITC-labeled homooligomer, FITC-S-dC20 and FIT-C-S-dT20, also accumulated in the nucleus of MOLT-4/HIV-1 cells, but weak fluorescence was observed on the cell membrane and in the cytoplasm of the FITC-S-random treated MOLT-4/HIV-1 and MOLT-4 cells.

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Documento generato il 18/09/20 alle ore 17:04:26