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Titolo:
EXPRESSION OF THE ESCHERICHIA-COLI FPG GENE IN MAMMALIAN-CELLS REDUCES THE MUTAGENICITY OF GAMMA-RAYS
Autore:
LAVAL F;
Indirizzi:
INST GUSTAVE ROUSSY,INSERM,U247,RADIOCHIM ADN GRP F-94805 VILLEJUIF FRANCE
Titolo Testata:
Nucleic acids research
fascicolo: 23, volume: 22, anno: 1994,
pagine: 4943 - 4946
SICI:
0305-1048(1994)22:23<4943:EOTEFG>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
APURINIC APYRIMIDINIC SITES; OXIDATIVE DNA DAMAGE; PLUS VISIBLE-LIGHT; ESCHERICHIA-COLI; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; IONIZING-RADIATION; MUTAGENESIS; GLYCOSYLASE; CHROMATIN; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
F. Laval, "EXPRESSION OF THE ESCHERICHIA-COLI FPG GENE IN MAMMALIAN-CELLS REDUCES THE MUTAGENICITY OF GAMMA-RAYS", Nucleic acids research, 22(23), 1994, pp. 4943-4946

Abstract

The E.coli fpg gene encodes the formamido-pyrimidine-DNA-glycosylase (FPG protein) which specifically removes the formamido-pyrimidine and C8-oxoGuanine residues from gamma-irradiated DNA. The fpg gene was ligated in the psV2 vector and transfected into the Chinese hamster CHO and V-79 cells. The transfected cells expressed a formamido-pyrimidine-DNA-glycosylase activity 30 to 40-fold over the constitutive level. The resistance of CHO and V-79 cells to the lethal effect of gamma-rays was similar in control and transfected cells. Furthermore CHO cells expressing the fpg gene had the same resistance to the lethal effect of hydrogen peroxide as control cells. However,the sensitivity to the mutagenic effect of gamma-rays, measured as B-thioguanine resistance, decreased both in CHO and V-79 transfected cells. Since the lethal effectof gamma-rays was not modified in cells overproducing the FPG protein, the results suggest that this protein protects the cells against themutagenic lesions formed by ionizing radiations, and among them C8-oxoguanine.

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Documento generato il 11/07/20 alle ore 14:33:41