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Titolo:
EFFECT OF LIPOPOLYSACCHARIDE ON THE PHYSICAL CONFORMATION OF THE ERYTHROCYTE CYTOSKELETAL PROTEINS
Autore:
BELLARY SS; ANDERSON KW; ARDEN WA; BUTTERFIELD DA;
Indirizzi:
UNIV KENTUCKY,DEPT CHEM ENGN LEXINGTON KY 40506 UNIV KENTUCKY,DEPT CHEM ENGN LEXINGTON KY 40506 UNIV KENTUCKY,CTR MEMBRANE SCI LEXINGTON KY 40506 UNIV KENTUCKY,DEPT SURG LEXINGTON KY 40506 UNIV KENTUCKY,DEPT CHEM LEXINGTON KY 40506
Titolo Testata:
Life sciences
fascicolo: 2, volume: 56, anno: 1994,
pagine: 91 - 98
SICI:
0024-3205(1994)56:2<91:EOLOTP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-SPIN-RESONANCE; BLOOD-CELL DEFORMABILITY; SEPTIC SHOCK; SEPSIS; MEMBRANES; SPECTRIN; SKELETAL; STATE; ASSOCIATION; SURFACE;
Keywords:
LIPOPOLYSACCHARIDE; SEPSIS; CYTOSKELETAL PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
S.S. Bellary et al., "EFFECT OF LIPOPOLYSACCHARIDE ON THE PHYSICAL CONFORMATION OF THE ERYTHROCYTE CYTOSKELETAL PROTEINS", Life sciences, 56(2), 1994, pp. 91-98

Abstract

Red blood cell deformability is important for effective circulation in the capillaries. It is known that red cell deformability is significantly reduced during septic shock. Surface to volume ratio, physical effects of the cytoskeletal proteins and the fluidity of lipid bilayer are some of the important intrinsic factors that regulate this mechanical function. Alterations in the physical conformation of cytoskeletalproteins in septic conditions could significantly alter their function. In this study, erythrocytes in whole blood were treated with lipopolysaccharide, the outer covering of Gram-negative bacteria released during Gram-negative sepsis. Electron paramagnetic resonance spectroscopy in conjunction with a protein-specific maleimide nitroxide spin label covalently bound to cytoskeletal proteins was used to investigate the resulting changes occurring in the physical state of cytoskeletal proteins in isolated membranes. Treatment of red blood cells with a lipopolysaccharide concentration as low as 40 mu g/mL of blood solution for 90 minutes showed a significant decrease in the relevant EPR parameter (p<0.01) of the spin label bound to subsequently isolated membranes, suggestive of a decreased segmental motion of the spin label and an increase in cytoskeletal protein-protein interactions. These results suggest a marked conformational alteration in the cytoskeletal proteinsinduced by the lipopolysaccharide and may explain, in part, the marked reduction in red blood cell deformability during septic shock. Bacterial lipopolysaccharide does not exert most of its effects on the hostdirectly, but rather elicits the production of host factors that leads to complex septic shock. Leukocytes, endothelial tissue and many other cells release these mediators. Leukocytes are thought to be a particularly important source of such mediators, including cytokines (tumornecrosis factor, interleukins, etc.), oxygen free radicals, proteases, and hydrolyses. In order to characterize the possible mechanism by which the lipopolysaccharide acts on the physical state of the erythrocyte cytoskeleton, erythrocytes void of leukocytes and plasma were treated with lipopolysaccharide. The relevant EPR parameter showed no significant change over the control value. These results indicate that theleukocytes and their factors are responsible for the rearrangements seen in the cytoskeletal proteins of the erythrocyte membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 05:51:06