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Titolo:
RAPID AND SELECTIVE CYCLIC VOLTAMMETRIC MEASUREMENTS OF EPINEPHRINE AND NOREPINEPHRINE AS A METHOD TO MEASURE SECRETION FROM SINGLE BOVINE ADRENAL-MEDULLARY CELLS
Autore:
PIHEL K; SCHROEDER TJ; WIGHTMAN RM;
Indirizzi:
UNIV N CAROLINA,DEPT CHEM,CB 3290 CHAPEL HILL NC 27599 UNIV N CAROLINA,DEPT CHEM CHAPEL HILL NC 27599
Titolo Testata:
Analytical chemistry
fascicolo: 24, volume: 66, anno: 1994,
pagine: 4532 - 4537
SICI:
0003-2700(1994)66:24<4532:RASCVM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIVIDUAL CHROMAFFIN CELLS; CATECHOLAMINE SECRETION; VESICLE FUSION; EXOCYTOSIS; ELECTRODES; RELEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
K. Pihel et al., "RAPID AND SELECTIVE CYCLIC VOLTAMMETRIC MEASUREMENTS OF EPINEPHRINE AND NOREPINEPHRINE AS A METHOD TO MEASURE SECRETION FROM SINGLE BOVINE ADRENAL-MEDULLARY CELLS", Analytical chemistry, 66(24), 1994, pp. 4532-4537

Abstract

Background-subtracted cyclic voltammetry at a scan rate of 800 V/s with carbon fiber microelectrodes has been used to detect and differentiate between epinephrine and norepinephrine. At very positive potentials (>1 V vs SSCE) in pH 7.4 aqueous buffer, a second oxidation wave is observed for epinephrine, a secondary amine. In contrast, the second oxidation wave is not observed for norepinephrine, a primary amine. Theamplitude of the second wave for epinephrine is enhanced when the wave form employed does not allow reduction of the electrogenerated o-quinone back to epinephrine. This indicates that the oxidation process atthe second wave must be preceded by adsorption of the o-quinone at the electrode surface. The temporal response of the method was investigated by iontophoretic ejection of catecholamine onto an electrode. The response time was found to be limited by the repetition rate of the cyclic voltammograms (16.7 ms in this work). This electrochemical technique was used to resolve catecholamine release from individual vesiclesof cultured bovine adrenal medullary cells. Most of the adrenal medullary cells released either epinephrine or norepinephrine, but 17% of the cells released mixtures of these two compounds. In these cells, each secretory vesicle appeared to contain either epinephrine or norepinephrine.

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Documento generato il 14/07/20 alle ore 13:18:01