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Titolo:
MOLECULAR-STRUCTURE AND ORGANIZATION OF THE HUMAN MANGANESE SUPEROXIDE-DISMUTASE GENE
Autore:
WAN XS; DEVALARAJA MN; STCLAIR DK;
Indirizzi:
UNIV KENTUCKY,GRAD CTR TOXICOL,210 FUNKHOUSER BLDG LEXINGTON KY 40506 UNIV KENTUCKY,GRAD CTR TOXICOL LEXINGTON KY 40506
Titolo Testata:
DNA and cell biology
fascicolo: 11, volume: 13, anno: 1994,
pagine: 1127 - 1136
SICI:
1044-5498(1994)13:11<1127:MAOOTH>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR NECROSIS FACTOR; NF-KAPPA-B; PROTEIN-KINASE-C; TRANSCRIPTION FACTOR; LUNG FIBROBLASTS; OXYGEN; CELLS; INDUCTION; ACTIVATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
X.S. Wan et al., "MOLECULAR-STRUCTURE AND ORGANIZATION OF THE HUMAN MANGANESE SUPEROXIDE-DISMUTASE GENE", DNA and cell biology, 13(11), 1994, pp. 1127-1136

Abstract

Human manganese superoxide dismutase (MnSOD) is one of the major cellular defense enzymes that protects against toxic effects of superoxideradicals. Overexpression of human MnSOD has been shown to inhibit radiation-induced neoplastic transformation, suppress malignancy of cancer cells, and increase tolerance to various toxic agents. To elucidate the human MnSOD gene structure for identification of potential regulatory elements, we isolated five lambda clones from a normal human genomic DNA library and sequenced the largest clone containing the entire human MnSOD gene. The results demonstrated that human MnSOD is a single-copy gene consisting of five exons interrupted by four introns with typical splice junctions. A distinctive transcription initiation site was identified 74 bp upstream from the translation start site. This transcription initiation site is preceded by a G + C-rich (78%) promoter region containing a cluster of seven SP1 and three AP2 consensus sequences with no TATA box or CAAT box. The 3'-flanking region of the MnSODgene contains one NF-KB consensus sequence. The presence of SP1, AP2,and NP-KB consensus sequences suggests that these potential regulatory elements may play a role in the regulation of human MnSOD gene expression.

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Documento generato il 02/10/20 alle ore 01:55:55