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Titolo:
CHARACTERIZATION AND ULTRASTRUCTURAL-LOCALIZATION OF A NOVEL 90-KDA PROTEIN UNIQUE TO SKELETAL-MUSCLE JUNCTIONAL SARCOPLASMIC-RETICULUM
Autore:
GUO W; JORGENSEN AO; CAMPBELL KP;
Indirizzi:
UNIV IOWA,COLL MED,DEPT PHYSIOL & BIOPHYS,HHMI,400 ECKSTEIN MED RES BLDG IOWA CITY IA 52242 UNIV IOWA,COLL MED,DEPT PHYSIOL & BIOPHYS,HHMI IOWA CITY IA 52242 UNIV IOWA,COLL MED,HOWARD HUGHES MED INST IOWA CITY IA 52242 UNIV TORONTO,DEPT ANAT TORONTO M5S 1A8 ON CANADA
Titolo Testata:
The Journal of biological chemistry
fascicolo: 45, volume: 269, anno: 1994,
pagine: 28359 - 28365
SICI:
0021-9258(1994)269:45<28359:CAUOAN>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-RELEASE CHANNEL; PURIFIED RYANODINE RECEPTOR; DEPENDENT CA-2+ CHANNEL; DIHYDROPYRIDINE RECEPTOR; 1,4-DIHYDROPYRIDINE RECEPTOR; BIOCHEMICAL-CHARACTERIZATION; GLYCOPROTEIN TRIADIN; TRANSVERSE TUBULES; FOOT PROTEIN; KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
W. Guo et al., "CHARACTERIZATION AND ULTRASTRUCTURAL-LOCALIZATION OF A NOVEL 90-KDA PROTEIN UNIQUE TO SKELETAL-MUSCLE JUNCTIONAL SARCOPLASMIC-RETICULUM", The Journal of biological chemistry, 269(45), 1994, pp. 28359-28365

Abstract

Monoclonal antibodies were used to identify and characterize a novel 90 kDa protein that was specifically localized to the junctional sarcoplasmic reticulum of rabbit skeletal muscle. Biochemical experiments show that the 90 kDa protein is an integral membrane protein of the junctional face membrane and is a substrate for the intrinsic protein kinase in triads. Immunofluorescence staining of serial transverse sections of skeletal muscle with a monoclonal antibody to the 90 kDa proteinshowed preferential staining of type II ''fast'' fibers. Specific labeling was confined to the interphase between the A- and I-bands, wherethe triad structure is localized. Immunoelectron microscopical labeling further indicates that the 90 kDa protein, like the ryanodine receptor/Ca2+-release channel and triadin, is confined to the terminal cisternae of the sarcoplasmic reticulum. Western blot analysis with a combination of monoclonal antibodies against the 90 kDa protein shows thatit is specifically expressed in skeletal muscle but not in cardiac muscle or brain. Similarly, specific immunofluorescence labeling to the 90 kDa protein was not detected in ventricular myocytes or vascular smooth muscle cells. The junctional localization and phosphorylation of this protein suggest that it may play an important regulatory or structural role in the skeletal muscle triad junction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 10:52:42