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Titolo:
CHARACTERIZATION OF A MEDIATED AMPEROMETRIC BIOSENSOR FOR HYPOXANTHINE USING DEFLAVO XANTHINE-OXIDASE
Autore:
ZHAO SS; LUONG JHT;
Indirizzi:
NATL RES COUNCIL CANADA,BIOTECHNOL RES INST MONTREAL H4P 2R2 PQ CANADA NATL RES COUNCIL CANADA,BIOTECHNOL RES INST MONTREAL H4P 2R2 PQ CANADA
Titolo Testata:
Electroanalysis
fascicolo: 10, volume: 6, anno: 1994,
pagine: 830 - 837
SICI:
1040-0397(1994)6:10<830:COAMAB>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYME ELECTRODES; PHOSPHATE; GLUCOSE; OXYGEN; SYSTEM;
Keywords:
XANTHINE OXIDASE; DEFLAVO XANTHINE OXIDASE; HYPOXANTHINE/XANTHINE; MEDIATED BIOSENSOR; MEDIATOR; 1,1'-DIMETHYLFERROCENE; 1,1'-DIMETHYLFERRICINIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
S.S. Zhao e J.H.T. Luong, "CHARACTERIZATION OF A MEDIATED AMPEROMETRIC BIOSENSOR FOR HYPOXANTHINE USING DEFLAVO XANTHINE-OXIDASE", Electroanalysis, 6(10), 1994, pp. 830-837

Abstract

Deflavo xanthine oxidase was prepared by removing the flavine adeninedinucleotide (FAD) group from the native enzyme using 2 M CaCl2. The deflavo enzyme was effectively stabilized by 5 mM EDTA in Tris buffer solution, pH 8, and retained about 80% of its activity after several days when stored in this medium at 4 degrees C. The deflavo enzyme was then used for the development of a mediated amperometric electrode forhypoxanthine/xanthine using 1,1'-dimethylferricinium as mediator, andis performance characteristics were compared to a native enzyme electrode. The resulting deflavo xanthine oxidase electrode was not noticeably sensitive to oxygen in the presence of hypoxanthine, while its capability to interact with ferricinium remained intact. The electrode sensor with immobilized deflavo enzyme sf;on ed high operational and storage stabilities with a detection limit of about 0.1 mu M. No significant sensitivity loss was observed on the enzyme electrode after 150 repeated analyses during a 7.5 hour operation. The deflavo enzyme electrode retained 80% of its sensitivity when stored at 4 degrees C after amonth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 17:58:49