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Titolo:
DEVELOPMENTAL REGULATION OF MULTIPLE NICOTINIC ACHR CHANNEL SUBTYPES IN EMBRYONIC CHICK HABENULA NEURONS - CONTRIBUTIONS OF BOTH THE ALPHA-2 AND ALPHA-4 SUBUNIT GENES
Autore:
BRUSSAARD AB; YANG X; DOYLE JP; HUCK S; ROLE LW;
Indirizzi:
COLUMBIA UNIV P&S,PSYCHIAT INST RES ANNEX,DEPT ANAT & CELL BIOL,CTR NEUROBIOL & BEHAV NEW YORK NY 10032 COLUMBIA UNIV P&S,PSYCHIAT INST RES ANNEX,DEPT ANAT & CELL BIOL,CTR NEUROBIOL & BEHAV NEW YORK NY 10032
Titolo Testata:
Pflugers Archiv
fascicolo: 1, volume: 429, anno: 1994,
pagine: 27 - 43
SICI:
0031-6768(1994)429:1<27:DROMNA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETYLCHOLINE-RECEPTOR CHANNELS; XENOPUS MYOCYTE CULTURE; PATCH-CLAMP TECHNIQUES; CENTRAL-NERVOUS-SYSTEM; CHOLINE-ACETYLTRANSFERASE; SYMPATHETIC NEURONS; GANGLION NEURONS; MESSENGER-RNA; IMMUNOHISTOCHEMICAL LOCALIZATION; MONOCLONAL-ANTIBODIES;
Keywords:
NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR; HABENULA COMPLEX; ANTISENSE OLIGONUCLEOTIDES; SINGLE-CHANNEL RECORDING; NEURONAL BUNGAROTOXIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
A.B. Brussaard et al., "DEVELOPMENTAL REGULATION OF MULTIPLE NICOTINIC ACHR CHANNEL SUBTYPES IN EMBRYONIC CHICK HABENULA NEURONS - CONTRIBUTIONS OF BOTH THE ALPHA-2 AND ALPHA-4 SUBUNIT GENES", Pflugers Archiv, 429(1), 1994, pp. 27-43

Abstract

Habenula neurons from both early and late stage embryonic chickens express multiple subtypes of nicotinic acetylcholine receptor channels (nAChRs). The channel subtypes expressed by habenula neurons are similar in functional properties, but apparently distinct in subunit composition, from their peripheral counterparts in autonomic ganglia. Early in development, nicotine activates four classes of neuronal bungarotoxin (nBGT)-sensitive channels (approx. conductance=15, 30, 50, 60pS) that are intermingled on the surface of habenula neuronal somata. In neurons removed from older animals, nAChR channel activity has increased 4- to 40-fold and channel subtypes have become spatially segregated from one another. Analysis of the profile of nAChR subunit gene expression by polymerase chain reaction indicates that several of the alpha-type subunit genes, including alpha 2,3,4,5,7, and alpha 8, as well as both beta 2 and beta 4, are expressed. Treatment of the neurons with subunit specific antisense oligonucleotides reveals that the alpha 2 and alpha 4 (but not alpha 3) subunits contribute to the functional profile of native nAChRs expressed by habenula neurons. Consideration of thefunctional properties and apparent subunit composition of autonomic ganglion nAChRs in the chick suggests that habenula neurons may utilizea very distinct set of subunit combinations to produce an array of nAChR channel subtypes similar in both conductance and pharmacological profile to those expressed by sympathetic neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:25:23