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Titolo:
CELLULAR-LOCALIZATION AND FUNCTIONAL-ANALYSIS OF THE PROTEIN ENCODED BY THE CHROMOSOMAL VIRULENCE GENE (ACVB) OF AGROBACTERIUM-TUMEFACIENS
Autore:
KANG HW; WIRAWAN IGP; KOJIMA M;
Indirizzi:
NAGOYA UNIV,CTR BIOSCI,CHIKUSA KU NAGOYA AICHI 46401 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 11, volume: 58, anno: 1994,
pagine: 2024 - 2032
SICI:
0916-8451(1994)58:11<2024:CAFOTP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TI-PLASMID; RHIZOBIUM-MELILOTI; PLANT-CELLS; GENE; MUTANTS; DNA; IDENTIFICATION; TRANSDUCTION; ATTACHMENT; SEQUENCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
H.W. Kang et al., "CELLULAR-LOCALIZATION AND FUNCTIONAL-ANALYSIS OF THE PROTEIN ENCODED BY THE CHROMOSOMAL VIRULENCE GENE (ACVB) OF AGROBACTERIUM-TUMEFACIENS", Bioscience, biotechnology, and biochemistry, 58(11), 1994, pp. 2024-2032

Abstract

A chromosomal virulence gene, acvB, of Agrobacterium tumefaciens [J. Bacterial., 175, 3208-3212 (1993)] was over-expressed in Escherichia coli. A 47-kDa protein was produced and localized in the periplasmic space of E. coli. Amino acid sequence analysis of its N-terminal demonstrated that a signal peptide of 24 amino acids was cleaved from the preAcvB protein to produce the mature 47-kDa protein. Western-blot analysis using the antiserum against the AcvB protein detected a 47-kDa protein in the periplasmic space only with strain A208 (acvB(+)). The amount of AcvB protein synthesized was not increased in strain A208 by induction with acetosyringone (100 mu M). There was observed no significant difference in induction by acetosyringone of virB::lacZ, virD::lacZ, and virE::lacZ fusion genes regardless of the presence or absence of the acvB gene. The T-strand (lower strand of T-DNA) was detected in str:ins A208 as well as B119 (acvB(-)) which were cultured in induction medium containing acetosyringone. AcvB protein bound to single-stranded DNAs with no apparent sequence specificity. The results suggest that AcvB protein binds to the T-strand in periplasm and mediates the transfer of the T-strand from A. tumefaciens to the host plant cell.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:04:02