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Titolo:
LECTIN-HISTOCHEMICAL ANALYSIS OF GLYCANS IN OVINE AND BOVINE NEAR-TERM PLACENTAL BINUCLEATE CELLS
Autore:
JONES CJP; KOOB B; STODDART RW; HOFFMANN B; LEISER R;
Indirizzi:
UNIV MANCHESTER,DEPT PATHOL SCI MANCHESTER M13 9PT LANCS ENGLAND UNIV GIESSEN,INST VET ANAT HISTOL & EMBRYOL D-35392 GIESSEN GERMANY UNIV GIESSEN,AMBULATOR & GEBURTSHILFL VET KLIN D-35392 GIESSEN GERMANY
Titolo Testata:
Cell and tissue research
fascicolo: 3, volume: 278, anno: 1994,
pagine: 601 - 610
SICI:
0302-766X(1994)278:3<601:LAOGIO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
WHEAT-GERM-AGGLUTININ; ACID-SPECIFIC LECTIN; SLUG LIMAX-FLAVUS; SIALIC-ACID; CHORIONIC SOMATOMAMMOTROPIN; MONOCLONAL-ANTIBODY; BIOLOGICAL-ACTIVITY; BINDING-SITES; SHEEP; LACTOGEN;
Keywords:
BINUCLEATE CELLS; PLACENTA; TROPHOBLAST; LECTINS; PREGNANCY; COW; SHEEP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
C.J.P. Jones et al., "LECTIN-HISTOCHEMICAL ANALYSIS OF GLYCANS IN OVINE AND BOVINE NEAR-TERM PLACENTAL BINUCLEATE CELLS", Cell and tissue research, 278(3), 1994, pp. 601-610

Abstract

Chorionic binucleate cells (BNC) occur in several ruminants includingcow, deer, goat and sheep. They migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. We have compared the BNC of near-term, resin-embedded, ovine and bovine placentae using 15 biotinylated lectins and an avidin-peroxidase revealing system. There was pronounced conservation of saccharides between the two species. Several sub-types of N-glycan were present, with highly branched structures being abundant, as shown by Galanthus nivalis, Pisum sativum and Phaseolus vulgaris (leuko) agglutinins. Among the non-reducing terminal saccharides conserved were GalNAc alpha 1,3(Fuc alpha 1,2)- Gal beta 1,4GlcNAc beta 1-, GalNAc alpha 1,6Gal beta 1-, Gal beta 1,4GlcNAc- and Gal beta 1,3GalNAc alpha 1- shown by Dolichos biflorus, Wister-in floribunda, Erythrina cristagalli, and Maclura pomifera agglutinins, respectively. Arachis hypogaea and Glycine max agglutinins tended to bind to bovine BNC at different stages of maturity, while fucosyl residues detectableby Tetragonolobus purpureus and Ulex europaeus-1 agglutinins were notobserved in either species. The only major difference related to sialyl residues, with alpha 2,3-linked sialic acid being present in bovine(Maackia amurensis, Limax flavus) and alpha 2,6 sialic acid being present in ovine (Sambucus nigra agglutinin) cells. This conservation of glycan may be related to glycosylation of peptide hormones in the granules, and may thus be important in the targeting of these hormones to their receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 15:49:59