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Titolo:
THE MYELOID-CELL-SPECIFIC C-FES PROMOTER IS REGULATED BY SP1, PU.1, AND A NOVEL TRANSCRIPTION FACTOR
Autore:
HEYDEMANN A; JUANG G; HENNESSY K; PARMACEK MS; SIMON MC;
Indirizzi:
UNIV CHICAGO,HOWARD HUGHES MED INST,5841 S MARYLAND AVE,MC 1028 CHICAGO IL 60637 UNIV CHICAGO,HOWARD HUGHES MED INST CHICAGO IL 60637 UNIV CHICAGO,DEPT MOLEC GENET & CELL BIOL CHICAGO IL 60637 UNIV CHICAGO,DEPT MED CHICAGO IL 60637
Titolo Testata:
Molecular and cellular biology
fascicolo: 4, volume: 16, anno: 1996,
pagine: 1676 - 1686
SICI:
0270-7306(1996)16:4<1676:TMCPIR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE-PROTEIN-KINASE; HL-60 LEUKEMIA-CELLS; EXPRESSION; DIFFERENTIATION; GENE; IDENTIFICATION; SEQUENCES; ONCOGENE; DOMAIN; BINDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
A. Heydemann et al., "THE MYELOID-CELL-SPECIFIC C-FES PROMOTER IS REGULATED BY SP1, PU.1, AND A NOVEL TRANSCRIPTION FACTOR", Molecular and cellular biology, 16(4), 1996, pp. 1676-1686

Abstract

The protein product of the c-fps/fes (c-fes) proto-oncogene has been implicated in the normal development of myeloid cells (macrophages andneutrophils), mRNA for c-fes has been detected exclusively in myeloidcells and vascular endothelial cells in adult mammals, Although a 13-kilobase-pair (kb) human c-fes transgene exhibits high levels of expression in mice, the sequences that confer myeloid-cell-specific expression of the human c-fes gene have not been defined, Transient-transfection experiments demonstrated that plasmids containing 446 bp of c-fes 5'-flanking sequences linked to a luciferase reporter gene were activeexclusively in myeloid cells, No other DNA elements within the 13-kb human c-fes locus contained positive cis-acting elements, with the exception of a weakly active region within the 3'-flanking sequences, DNase I footprinting assays revealed four distinct sites that bind myeloid nuclear proteins (-408 to -386, -293 to -254, -76 to -65, and -34 to+3), However, the first two footprints resided in sequences that werelargely dispensable for transient activity, Plasmids containing 151 bp of 5'-flanking sequences confer myeloid-cell-specific gene expression, Electrophoretic mobility shift analyses demonstrated that the 151-bp region contains nuclear protein binding sites for Sp1, PU.1, and/or Elf-1, and a novel factor, This unidentified factor binds immediately 3' of the PU.1/Elf-1 site and appears to be myeloid cell specific, Mutation of the PU.1/Elf-1 site or the 3' site (FP4-3') within the context of the c-fes promoter resulted in substantially reduced activity in transient transfections, Furthermore, transient-cotransfection assays demonstrated that PU.1 (and not Elf-1) can transactivate the c-fes promoter in nonmyeloid cell lines, We conclude that the human c-fes gene contains a strong myeloid-cell-specific promoter that is regulated by Sp1, PU.1, and a novel transcription factor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 08:53:41