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Titolo:
ANALYSIS OF MUSCLE CREATINE-KINASE GENE REGULATORY ELEMENTS IN SKELETAL AND CARDIAC MUSCLES OF TRANSGENIC MICE
Autore:
DONOVIEL DB; SHIELD MA; BUSKIN JN; HAUGEN HS; CLEGG CH; HAUSCHKA SD;
Indirizzi:
UNIV WASHINGTON,DEPT BIOCHEM,BOX 357350 SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SEATTLE WA 98195 BRISTOL MYERS SQUIBB PHARMACEUT RES INST SEATTLE WA 98121
Titolo Testata:
Molecular and cellular biology
fascicolo: 4, volume: 16, anno: 1996,
pagine: 1649 - 1658
SICI:
0270-7306(1996)16:4<1649:AOMCGR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAVY-CHAIN GENE; LOOP-HELIX PROTEINS; ACTIN GENE; DEVELOPMENTAL REGULATION; MOUSE MYOBLASTS; NUCLEAR FACTOR; I GENE; EXPRESSION; ENHANCER; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
79
Recensione:
Indirizzi per estratti:
Citazione:
D.B. Donoviel et al., "ANALYSIS OF MUSCLE CREATINE-KINASE GENE REGULATORY ELEMENTS IN SKELETAL AND CARDIAC MUSCLES OF TRANSGENIC MICE", Molecular and cellular biology, 16(4), 1996, pp. 1649-1658

Abstract

Regulatory regions of the mouse muscle creatine kinase (MCK) gene, previously discovered by analysis in cultured muscle cells, were analyzed in transgenic mice, The 206-bp MCK enhancer at nt -1256 was requiredfor high-level expression of MCK-chloramphenicol acetyltransferase fusion genes in skeletal and cardiac muscle; however, unlike its behavior in cell culture, inclusion of the l-kb region of DNA between the enhancer and the basal promoter produced a 100-fold increase in skeletal muscle activity, Analysis of enhancer control elements also indicated major differences between their properties in transgenic muscles and in cultured muscle cells, Transgenes in which the enhancer right E box or CArG element were mutated exhibited expression levels that were indistinguishable from the wild-type transgene, Mutation of three conserved E boxes in the MCK 1,256-bp 5' region also had no effect on transgene expression in thigh skeletal muscle expression, All of these mutations significantly reduced activity in cultured skeletal myocytes, However, the enhancer AT-rich element at nt -1195 was critical for expression in transgenic skeletal muscle, Mutation of this site reduced skeletal muscle expression to the same level as transgenes lacking the 206-bp enhancer, although mutation of the AT-rich site did not affect cardiac muscle expression, These results demonstrate clear differences between the activity of MCK regulatory regions in cultured muscle cells and in whole adult transgenic muscle. This suggests that there are alternative mechanisms of regulating the MCK gene in skeletal and cardiac muscle under different physiological states.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:31:05