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Titolo:
DETECTION OF ENDOGENOUS BETA-GLUCURONIDASE ACTIVITY IN ASPERGILLUS-NIGER
Autore:
GOTTSCHALK TE; NIELSEN JE; RASMUSSEN P;
Indirizzi:
DANISCO BIOTECHNOL,LANGEBROGADE 1,POB 17 DK-1001 COPENHAGEN K DENMARK
Titolo Testata:
Applied microbiology and biotechnology
fascicolo: 1-2, volume: 45, anno: 1996,
pagine: 240 - 244
SICI:
0175-7598(1996)45:1-2<240:DOEBAI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSION SIGNALS; FILAMENTOUS FUNGI; PLANTS; GENE; NIDULANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
T.E. Gottschalk et al., "DETECTION OF ENDOGENOUS BETA-GLUCURONIDASE ACTIVITY IN ASPERGILLUS-NIGER", Applied microbiology and biotechnology, 45(1-2), 1996, pp. 240-244

Abstract

An endogenous beta-glucuronidase that hydrolyses the chromogenic substrate 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-gluc) in Aspergillus niger is reported. The activity was induced when the fungus was grown in media containing xylan, but was either very low, or absent, when grown on glucose, Endogenous beta-glucuronidase was primarily located in newly formed hyphae, and was apparent at pH values between 3 and 6. Hydrolysis of X-gluc was sensitive to the inhibitor D-saccharic acid 1,4-lactone and was irreversibly inactivated by heating. The bacterial uidA beta-glucuronidase reporter gene was strongly expressed in the hyphae of transformed A. niger but, in contrast to the endogenous activity, the enzyme was also active at pH 7-8.5. Histochemical localization of uidA expression in A. niger, without interference from the endogenous beta-glucuronidase activity, was achieved by staining at thispH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 18:29:44