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Titolo:
MYOGENIC DETERMINATION FACTOR EXPRESSION IN THE DEVELOPING AVIAN LIMBBUD - AN RT-PCR ANALYSIS
Autore:
LINJONES J; HAUSCHKA SD;
Indirizzi:
UNIV WASHINGTON,DEPT BIOCHEM,BOX 357350 SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SEATTLE WA 98195 UNIV WASHINGTON,DEPT ZOOL SEATTLE WA 98195
Titolo Testata:
Developmental biology
fascicolo: 2, volume: 174, anno: 1996,
pagine: 407 - 422
SICI:
0012-1606(1996)174:2<407:MDFEIT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-FORMING CELLS; CLONAL ANALYSIS; VERTEBRATE MYOGENESIS; REGULATORY GENE; SKELETAL-MUSCLE; MOUSE EMBRYO; TRANSCRIPTION; MYOD1; SOMITE; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
J. Linjones e S.D. Hauschka, "MYOGENIC DETERMINATION FACTOR EXPRESSION IN THE DEVELOPING AVIAN LIMBBUD - AN RT-PCR ANALYSIS", Developmental biology, 174(2), 1996, pp. 407-422

Abstract

This study. examines the time of appearance of myogenic determinationfactor (MDF) transcripts in developing chick limbs and other embryonic tis sues using reverse transcription-polymerase chain reaction (RT -PCR). In this as say, PCR amplification profiles of cDNA samples fromstage 15-26 limb buds are compared to those of Ell heart, a tissue operationally defined as containing background levels of MDP transcriptswhich are thought to be nonfunctional. Myf 5 and MRF 4 transcripts are detected in stage 15 forelimbs, which is the earliest stage limb in which myogenic precursors have been detected following their migrationfrom the somite. In contrast, MyoD and myogenin transcripts do not appear until about 1.5 days later, closer to the time of cytological muscle differentiation. A survey of MyoD, myf 5, and MRF 4 transcripts inother embryonic tissues reveals that MyoD transcripts are distributedin a highly skeletal muscle-specific pattern. In contrast, myf 5 and MRP 4 mRNAs are detected at significant levels in embryonic tissues that do not contain muscle or muscle precursor cells such as stage 9-15 neural tubes and stage 9-12 lateral plates, while these transcripts are found at very low levels in Ell heart and liver. The RT-PCR results are compared to those from in situ hybridization experiments as well as from cell culture assays of the myogenic potential of early limb cells. (C) 1996, Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:14:27