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Titolo:
NUMA ASSEMBLES INTO AN EXTENSIVE FILAMENTOUS STRUCTURE WHEN EXPRESSEDIN THE CELL CYTOPLASM
Autore:
SAREDI A; HOWARD L; COMPTON DA;
Indirizzi:
DARTMOUTH COLL SCH MED,DEPT BIOCHEM HANOVER NH 03755 DARTMOUTH COLL SCH MED,DEPT BIOCHEM HANOVER NH 03755 DARTMOUTH COLL,RIPPEL ELECTRON MICROSCOPE FACIL HANOVER NH 03755
Titolo Testata:
Journal of Cell Science
, volume: 109, anno: 1996,
parte:, 3
pagine: 619 - 630
SICI:
0021-9533(1996)109:<619:NAIAEF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC APPARATUS PROTEIN; CULTURED-MAMMALIAN-CELLS; MONOCLONAL-ANTIBODIES; NUCLEAR MATRIX; SPN ANTIGEN; PTK2 CELLS; MITOSIS; SPINDLE; IDENTIFICATION; MICROINJECTION;
Keywords:
NUMA; NUCLEUS; MITOSIS; FILAMENT; NUCLEAR MATRIX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
A. Saredi et al., "NUMA ASSEMBLES INTO AN EXTENSIVE FILAMENTOUS STRUCTURE WHEN EXPRESSEDIN THE CELL CYTOPLASM", Journal of Cell Science, 109, 1996, pp. 619-630

Abstract

NuMA is a 236 kDa protein that participates in the organization of the mitotic spindle despite its strict localization in the nucleus during interphase. To test how cells progress through mitosis when NuMA is localized in the cytoplasm instead of the nucleus, we have deleted thenuclear localization sequence of NuMA using site-directed mutagenesisand transiently expressed this mutant protein (NuMA-Delta NLS) in BHK-21 cells. During interphase, NuMA-Delta NLS accumulates in the cytoplasm as a large mass approximately the same size as the cell nucleus. When cells enter mitosis, NuMA-Delta NLS associates normally with the mitotic spindle without causing any apparent deleterious effects on theprogression of mitosis. Examination of the cytoplasmic mass formed byNuMA-Delta NLS using transmission electron microscopy (TEM) revealed an extensive network of similar to 5 nm filaments that are further organized by the presence of dynamic microtubules into a dense web of solid, similar to 23 nm cables. Using how cytometry, we have isolated theintact filamentous mass formed by NuMA-Delta NLS from lysates of transiently transfected cells. These isolated structures are constructed of networks of interconnected 5 nn filaments and are composed exclusively of NuMA. These data demonstrate that NuMA is capable of assembling into an extensive filamentous structure supporting the possibility that NuMA serves a structural function either in the nucleus during interphase or at the polar ends of the mitotic spindle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 11:24:55