Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
KATANIN, THE MICROTUBULE-SEVERING ATPASE, IS CONCENTRATED AT CENTROSOMES
Autore:
MCNALLY FJ; OKAWA K; IWAMATSU A; VALE RD;
Indirizzi:
UNIV CALIF DAVIS,SECT MOLEC & CELLULAR BIOL,2310 STORER HALL DAVIS CA95616 KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA JAPAN UNIV CALIF SAN FRANCISCO,DEPT PHARMACOL SAN FRANCISCO CA 95616 UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED RES INST SAN FRANCISCO CA 95616
Titolo Testata:
Journal of Cell Science
, volume: 109, anno: 1996,
parte:, 3
pagine: 561 - 567
SICI:
0021-9533(1996)109:<561:KTMAIC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS EGG EXTRACTS; STABLE MICROTUBULES; MITOTIC SPINDLES; MITOSIS; PROTEIN; DYNAMICS;
Keywords:
MICROTUBULE; KATANIN; CENTROSOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
F.J. Mcnally et al., "KATANIN, THE MICROTUBULE-SEVERING ATPASE, IS CONCENTRATED AT CENTROSOMES", Journal of Cell Science, 109, 1996, pp. 561-567

Abstract

The assembly and function of the mitotic spindle involve specific changes in the dynamic properties of microtubules. One such change results in the poleward flux of tubulin in which spindle microtubules polymerize at their kinetochore-attached plus ends while they shorten at their centrosome-attached minus ends. Since free microtubule minus ends do not depolymerize in vivo, the poleward flux of tubulin suggests thatspindle microtubules are actively disassembled at or near their centrosomal attachment points. The microtubule-severing ATPase, katanin, has the ability actively to sever and disassemble microtubules and is thus a candidate for the role of a protein mediating the poleward flux of tubulin. Here we determine the subcellular localization of katanin by immunofluorescence as a preliminary step in determining whether katanin mediates the poleward flux of tubulin. We find that katanin is highly concentrated at centrosomes throughout the cell cycle. Katanin's localization is different from that of gamma-tubulin in that microtubules are required to maintain the centrosomal localization of katanin. Direct comparison of the localization of katanin and gamma-tubulin reveals that katanin is localized in a region surrounding the gamma-tubulin-containing pericentriolar region in detergent-extracted mitotic spindles. The centrosomal localization of katanin is consistent with the hypothesis that katanin mediates the disassembly of microtubule minus ends during poleward flux.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 06:04:55