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Titolo:
INDUCTION OF MATURE NEURONAL PROPERTIES IN IMMORTALIZED NEURONAL PRECURSOR CELLS FOLLOWING GRAFTING INTO THE NEONATAL CNS
Autore:
SHIHABUDDIN LS; BRUNSCHWIG JP; HOLETS VR; BUNGE MB; WHITTEMORE SR;
Indirizzi:
UNIV MIAMI,SCH MED,MIAMI PROJECT CURE PARALYSIS,1600 NW 10TH AVE,R-48MIAMI FL 33136 UNIV MIAMI,SCH MED,MIAMI PROJECT CURE PARALYSIS MIAMI FL 33136 UNIV MIAMI,SCH MED,NEUROSCI PROGRAM MIAMI FL 33136 UNIV MIAMI,SCH MED,DEPT NEUROL SURG MIAMI FL 33136 UNIV MIAMI,SCH MED,DEPT CELL BIOL & ANAT MIAMI FL 33136 UNIV MIAMI,SCH MED,DEPT PHYSIOL & BIOPHYS MIAMI FL 33136
Titolo Testata:
Journal of neurocytology
fascicolo: 2, volume: 25, anno: 1996,
pagine: 101 - 111
SICI:
0300-4864(1996)25:2<101:IOMNPI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYMPATHETIC NEURONS; DIFFERENTIATION FACTOR; HIPPOCAMPAL-NEURONS; PARKINSONS-DISEASE; CONDITIONED MEDIUM; TARGET REGULATION; DEFINED MEDIUM; SPINAL-CORD; RAT-BRAIN; TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
L.S. Shihabuddin et al., "INDUCTION OF MATURE NEURONAL PROPERTIES IN IMMORTALIZED NEURONAL PRECURSOR CELLS FOLLOWING GRAFTING INTO THE NEONATAL CNS", Journal of neurocytology, 25(2), 1996, pp. 101-111

Abstract

RN33B, a conditionally-immortalized neuronal cell line, survives and differentiates following grafting into the neocortex and hippocampus of adult and neonatal rat hosts. We have previously shown that these cells assume shapes characteristic of endogenous neurons at the integration site and persist up to 24 weeks post-grafting. In the present study we use electron microscopy and immunohistochemistry to characterize such cells. Differentiated RN33B cells were identical in size to endogenous neurons and their sizes depended on the specific location of integration. RN33B cells in the granule cell layer of the dentate gyrus and CA3 and CA1 pyramidal layers were 9.0, 15.3, and 12.6 mu m in diameter, respectively. Grafted RN33B cells received synapses from fibres of host origin. Differentiated cells expressed neuronal markers, but not glial markers. Some differentiated cells expressed glutamate both invitro and in vivo whereas undifferentiated cells did not. Grafted RN33B cells that differentiated with morphologies similar to CA3 pyramidal neurons and pyramidal cortical neurons expressed Py antigen, a neuronal marker that is differentially expressed in endogenous large pyramidal neurons of the cerebral cortex and large pyramids of hippocampal field CA3. This Py immunoreactivity was region-specific and corresponded to the endogenous pattern of Py immunostaining. Collectively, these data indicate that RN33B cells are capable of region-specific differentiation and have the potential to integrate functionally into the hostCNS.

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Documento generato il 19/09/20 alle ore 09:31:39