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Titolo:
MUTANTS OF EUKARYOTIC INITIATION-FACTOR EIF-4E WITH ALTERED MESSENGER-RNA CAP-BINDING SPECIFICITY REPROGRAM MESSENGER-RNA SELECTION BY RIBOSOMES IN SACCHAROMYCES-CEREVISIAE
Autore:
VASILESCU S; PTUSHKINA M; LINZ B; MULLER PP; MCCARTHY JEG;
Indirizzi:
GESELL BIOTECHNOL FORSCH MBH,NATL BIOTECHNOL RES CTR,DEPT GENE EXPRESS,MASCHERODER WEG 1 D-38124 BRAUNSCHWEIG GERMANY GESELL BIOTECHNOL FORSCH MBH,NATL BIOTECHNOL RES CTR,DEPT GENE EXPRESS D-38124 BRAUNSCHWEIG GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 12, volume: 271, anno: 1996,
pagine: 7030 - 7037
SICI:
0021-9258(1996)271:12<7030:MOEIEW>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-FREE SYSTEM; RNA 5' CAP; MESSENGER-RNA; PROTEIN-SYNTHESIS; SECONDARY STRUCTURE; ESCHERICHIA-COLI; TRANSLATION; YEAST; EXPRESSION; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
S. Vasilescu et al., "MUTANTS OF EUKARYOTIC INITIATION-FACTOR EIF-4E WITH ALTERED MESSENGER-RNA CAP-BINDING SPECIFICITY REPROGRAM MESSENGER-RNA SELECTION BY RIBOSOMES IN SACCHAROMYCES-CEREVISIAE", The Journal of biological chemistry, 271(12), 1996, pp. 7030-7037

Abstract

Recognition of the 5'-end of eukaryotic mRNA by the ribosomal 43 S preinitiation complex involves the eukaryotic translation initiation factor elF-4E (eIF-4 alpha). Deletion mutants of the eIF-4E gene of Saccharomyces cerevisiae (CDC33) encoded proteins with reduced affinity forthe 5'-cap. One of these mutant proteins lacked any detectable binding to a cap analogue binding column, yet was still able to support cellgrowth. More than 17% of the total eIF-4E amino acid sequence could be removed without fully inactivating this factor, At least 30 of the N-terminal amino acids are not essential for function, The minimal functional eIF-4E protein segment therefore comprises at most 176 amino acids. The translation and growth defects of the deletion mutants could be at least partially compensated by increases in eIF-4E synthesis, possibly due to a mass-action effect on mRNA binding. Electroporation ofyeast spheroplasts with in vitro synthesized mRNA allowed us to characterize the ability of elF-4E mutant strains to distinguish between capped and uncapped mRNAs in vivo. Our data show that the cap specificity of eIF-4E determines to what extent the translational apparatus differentiates between capped and uncapped mRNAs and indicate the minimum relative mRNA (cap) binding activity of eIF-4E required for yeast cellviability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 23:46:41