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Titolo:
THE N-TERMINAL HALF OF EBNA2, EXCEPT FOR 7 PROLINES, IS NOT ESSENTIALFOR PRIMARY B-LYMPHOCYTE GROWTH TRANSFORMATION
Autore:
YALAMANCHILI R; HARADA S; KIEFF E;
Indirizzi:
HARVARD UNIV,BRIGHAM & WOMENS HOSP,DEPT MICROBIOL,75 FRANCIS ST BOSTON MA 02115 HARVARD UNIV,BRIGHAM & WOMENS HOSP,DEPT MICROBIOL BOSTON MA 02115 HARVARD UNIV,BRIGHAM & WOMENS HOSP,DEPT MOLEC GENET BOSTON MA 02115
Titolo Testata:
Journal of virology
fascicolo: 4, volume: 70, anno: 1996,
pagine: 2468 - 2473
SICI:
0022-538X(1996)70:4<2468:TNHOEE>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR-VIRUS; NUCLEAR ANTIGEN-2; BURKITTS-LYMPHOMA; EXPRESSION; TRANSACTIVATION; INFECTION; PROTEIN-2; ELEMENT; CELLS; CD23;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
R. Yalamanchili et al., "THE N-TERMINAL HALF OF EBNA2, EXCEPT FOR 7 PROLINES, IS NOT ESSENTIALFOR PRIMARY B-LYMPHOCYTE GROWTH TRANSFORMATION", Journal of virology, 70(4), 1996, pp. 2468-2473

Abstract

Previous molecular genetic analyses of Epstein-Barr virus nuclear protein 2 (EBNA2) identified a negative effect of deletion of codons 19 to 33 on transformation and gene transactivation, while deletion of codons 19 to 110 was a null mutation for transformation and gene transactivation. We here report the surprising finding that codons 2 to 88, which encode the highly conserved unique N terminus (amino acids 1 to 58) and most of the polyproline repeat (amino acids 59 to 95), can be deleted with only minimal effects on transformation. Codons 97 to 122 can also be deleted with only minimal effects on transformation. However, deletion of 35 of the 37 prolines (amino acids 59 to 93) or deletionof codons 2 to 95 results in a null transforming phenotype. Although EBNA2 from which codons 59 to 93 were deleted was a null mutation for transformation, it was similar to some transforming mutants of EBNA2 in abundance, in interaction with RBPJK, and in transactivation of the LMP1 promoter in transient transfection assays. These data indicate that between three and seven prolines are critical for EBNA2 structure or for intermolecular interaction. Aside from these seven prolines, codons encoding the rest of the N-terminal half (amino acids 2 to 230) ofEBNA2 are nonessential for primary B-lymphocyte growth transformation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:43:46