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Titolo:
HOMOLOGOUS DESENSITIZATION OF HUMAN CORTICOTROPIN-RELEASING FACTOR(1)RECEPTOR IN STABLE TRANSFECTED MOUSE FIBROBLAST CELLS
Autore:
DIETERICH KD; GRIGORIADIS DE; DESOUZA EB;
Indirizzi:
NEUROCRINE BIOSCI INC,3050 SCI PK RD SAN DIEGO CA 92121 NEUROCRINE BIOSCI INC SAN DIEGO CA 92121 UNIV CALIF SAN DIEGO,DEPT NEUROSCI SAN DIEGO CA 92037
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 710, anno: 1996,
pagine: 287 - 292
SICI:
0006-8993(1996)710:1-2<287:HDOHCF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTERIOR-PITUITARY-CELLS; BETA-ADRENERGIC-RECEPTOR; CYCLIC-AMP ACCUMULATION; FACTOR CRF RECEPTORS; AUTORADIOGRAPHIC LOCALIZATION; ADENYLATE-CYCLASE; TUMOR CELLS; RESPONSES; STRESS; ADRENALECTOMY;
Keywords:
ADENYLATE CYCLASE ACTIVITY; ADRENOCORTICOTROPIN; CAMP PRODUCTION; CORTICOTROPIN-RELEASING FACTOR; PITUITARY; RECEPTOR DOWN-REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
K.D. Dieterich et al., "HOMOLOGOUS DESENSITIZATION OF HUMAN CORTICOTROPIN-RELEASING FACTOR(1)RECEPTOR IN STABLE TRANSFECTED MOUSE FIBROBLAST CELLS", Brain research, 710(1-2), 1996, pp. 287-292

Abstract

Previous radioligand binding and second messenger studies have shown that corticotropin-releasing factor (CRF) modulates its receptor following both in vivo and in vitro treatment. In the present study, we determined the sequence of events leading to CRF-induced downregulation and desensitization of cloned CRF receptors in murine fibroblast cells (Ltk(-)) stably transfected with CRF(1) DNA (from human pituitary). Treatment of cells with rat/human CRF produced a dose- and time-dependent decrease in [I-125]Tyr(0)-ovine CRF ([I-125]oCRF) binding and a concomitant decrease in CRF-stimulated adenylate cyclase activity. Significant decreases in [I-125]oCRF binding and agonist-stimulated cAMP production were evident minutes after CRF treatment with maximal (60-80%) reductions seen following 1 h of CRF treatment. Scatchard analysis revealed that the decrease in [I-125]oCRF binding was due to the downregulation of the receptor with no significant alteration seen in the affinity of the ligand. Since the transfected cell line is engineered using an artificial promoter, we did not detect any significant changes inCRF(1) receptor mRNA levels following CRF treatment for up to 24 h.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 01:00:50